Reliability Evaluation of MALDI-TOF MS Associated with SARAMIS Software in Rapid Identification of Thermophilic Campylob
- PDF / 433,515 Bytes
- 5 Pages / 595.276 x 790.866 pts Page_size
- 29 Downloads / 166 Views
Reliability Evaluation of MALDI-TOF MS Associated with SARAMIS Software in Rapid Identification of Thermophilic Campylobacter Isolated from Food Graziella Ziino 1 & Stefania Maria Marotta 1 & Filippo Giarratana 1
&
Alessandro Giuffrida 1 & Felice Panebianco 2
Received: 19 October 2018 / Accepted: 21 January 2019 # Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract Thermophilic Campylobacter species represent the most frequent cause of food-borne disease worldwide. Biochemical and serological tests, according to ISO 10272:2017, are routinely used in microbiology laboratories for their detection/identification. However, these procedures require a lot of time and can incur in diagnostic mistakes. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is considered a promising identification method for bacteria and fungi from clinical isolates. The aim of this study was to evaluate the reliability of MALDI-TOF MS associated with SARAMIS software, in rapid identification of food-related Campylobacter spp., considering that this method was tested only on human and animal clinical strains. A total of 276 putative thermophilic Campylobacter strains, isolated from different foodstuffs according to ISO 10272-1:2006, were identified comparing the results of MALDI-TOF MS/SARAMIS with Multiplex PCR assay. MALDI-TOF MS/SARAMIS correctly identified 96% (n = 267) of strains at the species level, while only 2.5% (n = 7) of strains were not identified. Misidentification occurred in 0.7% (n = 2) of samples. The performance of this method demonstrated an overall sensitivity (Se) of 97.45% and specificity (Sp) of 100%. Thus, this method can be used for the rapid identification of Campylobacter strains isolated from food samples according to ISO 10272:2017. Keywords MALDI-TOF MS . SARAMIS TM . Campylobacter . Rapid identification . Food strains
Introduction Campylobacteriosis is the most commonly diagnosed gastrointestinal disease worldwide. In 2016, a total of 246,307 confirmed human cases were reported in the European Union (EFSA (European Food Safety Authority) and ECDC (European Centre for Disease Prevention and Control) 2017). The thermophilic Campylobacter species (C. jejuni, C. coli, and C. lari) are among the major bacterial causes of food-borne gastroenteritis including diarrhea, fever, and abdominal pain (Deckert et al. 2010). Infection may be serious on its own, but in some cases, it can also have long-term consequences such as
* Filippo Giarratana [email protected] 1
Department of Veterinary Sciences, University of Messina, Polo Universitario dell’Annunziata, 98168 Messina, Italy
2
Department of Agraria, BMediterranea^ University of Reggio Calabria, Feo di Vito, 89122 Reggio Calabria, Italy
reactive arthritis and Guillain–Barré syndrome, inflammatory bowel disease, and irritable bowel syndrome (Janssen et al. 2008). Campylobacter spp. are widespread in nature and the principal reservoirs is the intestinal tract of domesticated and wild mammals and bir
Data Loading...
