Extension and application of a database for the rapid identification of Vibrio using MALDI-TOF MS
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Extension and application of a database for the rapid identification of Vibrio using MALDI-TOF MS Jing Wu1, Yuexia Zhou2, Xiaofei Liu1, Yuan Cao1, Chengjin Hu1, Yingjian Chen1* 1 Departments of Laboratory Medicine, The 960th Hospital of the PLA Joint Logistics Support Force, Jinan 250031,
China 2 Teaching and Research Office of Medical Laboratory Science, Heze Medical College, Heze 274000, China
Received 21 October 2019; accepted 13 December 2019 © Chinese Society for Oceanography and Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract
Rapid and accurate identification of Vibrio species has been problematic because phenotypic characteristics are variable within species and biochemical identification requires two or more days to complete. Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has become a powerful tool for rapidly distinguishing between related bacterial species. However, its accuracy depends on the number of strains in a database. In the current study, we extend and apply the Vibrio database based on MALDI-TOF MS. A total of 74 strains of Vibrio representing 28 species were identified and included in new database. A phylogenetic tree based on rpoB sequence and dendrograms were constructed. We analyzed 30 clinical Vibrio of three species to evaluate the new database and carried out PCA dendrogram analyses for differences of strains. We created a new database that offered fast and accurate Vibrio identification. MSP and PCA dendrogram analyses provided technical support to track sources and incidences of Vibrio infection. In addition, the discovery of characteristic and differential peaks is useful for the future identification of Vibrio. This represents a powerful tool for the rapid and accurate classification and identification of Vibrio and closely related species. Key words: database, Vibrio, MALDI-TOF MS, rpoB, dendrograms Citation: Wu Jing, Zhou Yuexia, Liu Xiaofei, Cao Yuan, Hu Chengjin, Chen Yingjian. 2020. Extension and application of a database for the rapid identification of Vibrio using MALDI-TOF MS. Acta Oceanologica Sinica, 39(10): 140–146, doi: 10.1007/s13131-020-1635-8
1 Introduction Vibrio, one of the most common Gram-negative bacteria in the marine environment, is widely distributed on body surfaces, in the intestinal tracts of marine organisms and in seawater (Kaneko and Colwell, 1974). More than 20 Vibrio species infect fish or humans, causing serious diseases (Tiruvayipati and Bhassu, 2016; Cheng et al., 2015). For instance, V. vulnificus, V. parahaemolyticus, V. fluvialis and V. alginolyticus reportedly cause severe gastroenteritis, severe skin-soft tissue infections, septicemia, and other serious infections, particularly in immunocompromised hosts (Cheng et al., 2015). At present, methods to identify Vibrio are primarily based on microscopy and biochemical tests, which are time and labor-intensive as well as subjective, making it difficult to rapidly diagnose these pathogenic bacteria. Therefore, it is imperative to ident
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