Research on the regulatory mechanisms of self-incompatibility plasticity in Nicotiana alata
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Research on the regulatory mechanisms of selfincompatibility plasticity in Nicotiana alata Jugou Liao . Xuemei Wei . Zhiyun Chen . Xiaojun Qin . Zihui Pan . Yongzhi Niu . Wenlong Suo . Yunye Zheng . Wenguang Ma . Suiyun Chen
Received: 22 November 2019 / Accepted: 21 August 2020 / Published online: 2 September 2020 Ó Springer Nature B.V. 2020
Abstract Nicotiana alata is a model species used to study gametophytic self-incompatibility (SI), controlled by the S-locus (encoding S-RNase and SLF proteins expressed in pistil and pollen, respectively) as well as by modifier genes (such as 120 kDa, HT-b and Trxh). A previous study revealed that SI in N. alata accession S2S2 was plastic, and that SI was leaky with senescence of the pistil and plant. Here, we further explored its regulatory mechanisms. Reverse transcription quantitative PCR (RT-qPCR) and western
Jugou Liao and Xuemei Wei contributed equally to this work. J. Liao X. Wei Z. Chen X. Qin Z. Pan S. Chen (&) School of Ecology and Environmental Sciences, Biocontrol Engineering Research Center of Plant Diseases & Pests, Biocontrol Engineering Research Center of Crop Diseases & Pests, Yunnan University, Kunming 650091, Yunnan Province, China e-mail: [email protected] X. Wei School of Life Science, Dali University, Dali 671000, Yunnan Province, China Y. Niu W. Suo Yuxi Zhongyan Tobacco Seed Co., Ltd, 653100 Yuxi, China Y. Zheng W. Ma (&) Yunnan Academy of Tobacco Agricultural Sciences, 653100 Yuxi, China e-mail: [email protected]
blot results showed that during the senescence of pistil in S2S2, 120 kDa and HT-b were transcribed and translated at lower levels, while marked residual S-RNase activity was detected. The transcripts of Trxh varied irregularly. Only the transcription and translation of 120 kDa declined with the plant senescence. According to the results, PSF in S2S2 may be associated with down-regulated expression of 120 kDa and HT-b, while S-RNase and Trxh seem to be unrelated to this phenotype. These findings deepen our understanding of the regulatory mechanisms of SI plasticity in N. alata. Keywords Nicotiana alata Plasticity of selfincompatibility 120 kDa HT-b S-RNase activity
Introduction Self-incompatibility (SI) is widespread in plants, and acts to promote outcrossing and therefore prevent inbreeding depression (McClure 2006). According to the genetic control of the incompatibility phenotype in pollen, SI is classified as gametophytic self-incompatibility (GSI) and sporophytic self-incompatibility (SSI) (Kaufmann et al. 1992; Pandey 1960). GSI is the most abundant in flowering plants, and is distributed in all major clades of the monocots and dicots (Vieira et al. 2008; Steinbachs and Holsinger 2002). GSI can
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be further distributed into two types at the molecular level, namely, the S-RNase-based system in Solanaceae, Plantaginaceae and Rosaceae, and the Ca2?dependent system of the Papaveraceae (McClure and Franklin-Tong 2006). The specificity of Sol
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