Resveratrol-induced apoptosis is associated with regulating the miR-492/CD147 pathway in malignant melanoma cells
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ORIGINAL ARTICLE
Resveratrol-induced apoptosis is associated with regulating the miR-492/CD147 pathway in malignant melanoma cells Shuang Zhao 1,2 & Ling Tang 2,3 & Wangqing Chen 1,2 & Juan Su 1,2 & Fangfang Li 1,2 & Xiang Chen 1,2 & Lisha Wu 1,2,4,5 Received: 6 February 2020 / Accepted: 23 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Resveratrol (RES) as a natural phytoalexin has anti-tumor effects on various cancers through its pro-apoptotic activities. Our aim was to determine that RES induces apoptosis in melanoma cells by regulating miR-492 resulting in decreased CD147 expression. We treated A375 and SK-MEL-28 melanoma cells via RES at different concentrations and time-points. The results have shown that the inhibition rate of A375 and SK-MEL-28 was significantly increased after RES treatment. Subsequently, we investigated cell apoptosis by flow cytometry, as well as detected apoptotic-associated proteins including PARP, Caspase-3, Bcl-2, and Bax by western blotting. Meanwhile, the expression of miR-492 and CD147 was analyzed. We found that RES remarkably induces apoptosis in melanoma cells, along with an upregulation of miR-492 and the inhibition of CD147 expression. Furthermore, the detection of luciferase reporter activity confirmed that miR-492 could target CD147 mRNA, and transfected with mimic miR492 in cells reduced CD147 expression. We also performed the rescued experiment by using a miR-492 inhibitor in melanoma cells. The results showed that the ability of induced apoptosis by RES in melanoma cells was to be attenuated via inhibiting miR492 expression resulting in CD147 augment. Finally, we determined that the effect of RES-induced apoptosis in melanoma cells is associated with, at least in part, its ability to regulate the miR-492/CD147 pathway. Keywords Melanoma . Resveratrol . CD147 . miR-492 . Apoptosis
Introduction As a natural phytoalexin, resveratrol (3,4′,5-trihydroxystilbene) is produced by a limited number of plant species, such as red
Shuang Zhao and Ling Tang contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00210-020-01981-4) contains supplementary material, which is available to authorized users. * Lisha Wu [email protected] 1
Department of Dermatology, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha 410008, Hunan, China
2
Hunan Key Laboratory of Skin Cancer and Psoriasis, Changsha 410008, Hunan, China
3
Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China
4
Institute of Medical Sciences, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China
5
National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China
grapes (Zhang et al. 2006). Resveratrol (RES) has multiple biological functions, including anti-oxidative (AlBasher et al. 2020; Abdel-Daim et al. 2019), anti-inflammatory (Ibrahim et al
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