Retention Using Selective Hooks-Synchronized Secretion to Measure Local Exocytosis
Proteins destined to be exposed to the extracellular space enter the secretory pathway at the level of the endoplasmic reticulum. Proteins are then transported to the Golgi apparatus and addressed to their destination compartment, such as the plasma membr
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Florence Niedergang Nicolas Vitale Stéphane Gasman Editors
Exocytosis and Endocytosis Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, UK
For further volumes: http://www.springer.com/series/7651
For over 35 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-bystep fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice. These hallmark features were introduced by series editor Dr. John Walker and constitute the key ingredient in each and every volume of the Methods in Molecular Biology series. Tested and trusted, comprehensive and reliable, all protocols from the series are indexed in PubMed.
Exocytosis and Endocytosis Methods and Protocols
Edited by
Florence Niedergang Department of Infection, Immunity & Inflammation, Institut Cochin (Institut National de la santé et de la recherche médicale, Centre National de la Recherche Scientifique and Université de Paris), Paris, France
Nicolas Vitale Centre National de la Recherche Scientifique, Institut des Neurosciences Cellulaires et Intégratives, Université de Strasbourg, Strasbourg, France
Stéphane Gasman Centre National de la Recherche Scientifique, Institut des Neurosciences Cellulaires et Intégratives, Université de Strasbourg, Strasbourg, France
Editors Florence Niedergang Department of Infection, Immunity & Inflammation Institut Cochin (Institut National de la sante´ et de la recherche me´dicale, Centre National de la Recherche Scientifique and Universite´ de Paris) Paris, France
Nicolas Vitale Centre National de la Recherche Scientifique Institut des Neurosciences Cellulaires et Inte´gratives Universite´ de Strasbourg Strasbourg, France
Ste´phane Gasman Centre National de la Recherche Scientifique Institut des Neurosciences Cellulaires et Inte´gratives Universite´ de Strasbourg Strasbourg, France
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-0716-1043-5 ISBN 978-1-0716-1044-2 (eBook) https://doi.org/10.1007/978-1-0716-1044-2 © Springer Science+Business Media, LLC, part of Springer Nature 2021 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by si
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