RETRACTED ARTICLE: LncRNA TUG1 was upregulated in osteoporosis and regulates the proliferation and apoptosis of osteocla
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(2019) 14:416
RESEARCH ARTICLE
Open Access
LncRNA TUG1 was upregulated in osteoporosis and regulates the proliferation and apoptosis of osteoclasts Ye Han1, Chunying Liu2, Ming Lei1, Shaosong Sun1, Wenkui Zheng1, Yanan Niu1 and Xi Xia3*
Abstract Background: Long non-coding RNA (LncRNA) TUG1 plays critical roles in the development of human cancers. Its inhibition has been proved to participate in ankylosing spondylitis, which is an inverse pathological procedure of osteoporosis. In the present study, we aim to investigate the role of lncRNA TUG1 in ankylosing spondylitis. Materials and methods: Expressions of lncRNA TUG1 in plasma of 98 patients with osteoporosis and 60 healthy participants were detected by real-time quantitative PCR (RT-qPCR). Diagnostic values of lncRNA CASC11 for osteoclasts were performed by the ROC curve with osteoporosis patients as positive and healthy participants as negative. All experiments were repeated 3 times. Mean ± standard deviation was calculated. Results: We found that plasma lncRNA TUG1 was upregulated in osteoporosis patients than in healthy participants. Upregulation of plasma lncRNA TUG1 distinguished osteoporosis patients from healthy participants. LncRNA TUG1 level increased with the advances of clinical stages. Over-expression of lncRNA TUG1 promoted the proliferation and inhibited the apoptosis of mice osteoclasts, while lncRNA TUG1 siRNA silencing played an opposite role. In addition, lncRNA TUG1 over-expression led to downregulated PTEN, while lncRNA TUG1 siRNA silencing played an opposite role. Conclusion: Therefore, lncRNA TUG1 is upregulated in osteoporosis and regulates the proliferation and apoptosis of osteoclasts. lncRNA TUG1 knockdown may serve as a promising therapeutic target for osteoporosis by inhibiting the proliferation and promoting the apoptosis of osteoclasts through PTEN. Keywords: Osteoporosis, lncRNA TUG1, Osteoclast, Proliferation, Apoptosis
Background Osteoporosis is a bone disease which occurs from the imbalance between bone formation and resorption. The incidence rate of osteoporosis is higher in women than in men. Family history of fracture, low BMI, aging, and smoking are proven to be risk factors for osteoporosis in women [1]. However, a portion of the males, such as the ones with obesity, are also at high risk for osteoporosis [2]. Patients with osteoporosis are usually treated with calcium supplements and hormone replacement [3, 4]. However, therapeutic outcomes are generally unsatisfied due to adverse side effects or poor patient compliance. * Correspondence: [email protected] 3 Department of Orthopaedics, Baoding First Central Hospital, No. 320, Great Wall North Street, Baoding City 071000, Hebei Province, People’s Republic of China Full list of author information is available at the end of the article
Therefore, improvement in the treatment of osteoporosis is quite critical. Long non-coding RNAs (lncRNAs) are a subgroup of non-protein coding RNAs with lengths longer than 200 nucleotides [5]. Growing amounts of literature have shown that
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