LncRNA MIR4435-2HG is downregulated in osteoarthritis and regulates chondrocyte cell proliferation and apoptosis
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(2019) 14:247
RESEARCH ARTICLE
Open Access
LncRNA MIR4435-2HG is downregulated in osteoarthritis and regulates chondrocyte cell proliferation and apoptosis Yu Xiao1*† , Yucheng Bao2†, Liang Tang2 and Li Wang2
Abstract Purpose: MIR4435-2HG is an oncogenic lncRNA in gastric cancer and lung cancer. Our preliminary microarray data showed that MIR4435-2HG was downregulated in osteoarthritis plasma specimen, indicating the possible involvement of MIR4435-2HG in osteoarthritis. Results: MIR4435-2HG was downregulated in plasma of osteoarthritis than in plasma of healthy controls. Reduced levels of MIR4435-2HG expression effectively distinguished osteoarthritis patients from the control group. Expression levels of MIR4435-2HG increased after treatment. Overexpression of MIR4435-2HG promoted, while MIR4435-2HG knockdown inhibited the proliferation of chondrocytes. In contrast, MIR4435-2HG overexpression inhibited, while MIR4435-2HG knockdown promoted the apoptosis of chondrocytes. Conclusion: MIR4435-2HG is downregulated in osteoarthritis and regulates chondrocyte cell proliferation and apoptosis. Keywords: Osteoarthritis, lncRNA MIR4435-2HG, Proliferation, Apoptosis
Introduction Osteoarthritis (OA) is a common clinical degenerative joint disease that is characterized by subchondral bone thickening, degradation of articular cartilage, and the formation of osteophytes [1]. Osteoarthritis now has become a major problem of public health worldwide, and more than half of people aged older than 65 years are suffering from this disease [2]. OA is associated and is closely correlated with homeostatic imbalance, which results from aging [3]. With the increase in people’s life expectancy, the incidence of osteoarthritis is predicted to be further increased in the near future [4]. Although several risk factors have been characterized for osteoarthritis, the pathogenesis of this disease is still unclear and definitive cure is also not available [5]. Although long non-coding RNAs (lncRNAs) have no protein-coding capacity, there are key players in the development of human diseases [6, 7]. We carried out a * Correspondence: [email protected] † Yu Xiao and Yucheng Bao contributed equally to this work. 1 Department of Orthopaedic Surgery, Tianjin Hospital, No.406 Jiefang South Road, Hexi, Tianjin City 300211, People’s Republic of China Full list of author information is available at the end of the article
genome-wide transcriptome analysis of differentially expressed gene in plasma of osteoarthritis patients. Our preliminary data showed that MIR4435-2HG was downregulated in osteoarthritis (data not shown). MIR4435-2HG has been proved to be an oncogenic lncRNA in gastric cancer and lung cancer [8, 9]. We showed that MIR4435-2HG was downregulated in osteoarthritis and regulated chondrocyte cell proliferation and apoptosis.
Materials and methods Research subjects
A total of 78 osteoarthritis (44 cases of knee joint and 34 cases of hip joint; 40 males and 38 females; 60 to 78 years old; mean age 69.0 ± 6.0 years) patients and 58
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