RETRACTED ARTICLE: Long non-coding RNA LBX2-AS1 enhances glioma proliferation through downregulating microRNA-491-5p
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Cancer Cell International Open Access
PRIMARY RESEARCH
Long non-coding RNA LBX2-AS1 enhances glioma proliferation through downregulating microRNA-491-5p Qunbang Chen1, Jian Gao1, Yingjia Zhao2 and Ruizhe Hou1*
Abstract Background: Dysregulation of lncRNAs is frequent in glioma and has emerged as an important mechanism involved in tumorigenesis. Previous analysis of Chinese Glioma Genome Atlas (CGGA) database indicated that LBX2-AS1 expression is one of differentially expression lncRNA between lower grade glioma (LGG) (grade II and III) and glioblastoma multiforme (GBM). However, the function and mechanism of LBX2-AS1 in glioma has not been evaluated yet. Methods: Here, we analyzed the expression of LBX2-AS1 in GTEx data (normal brain), TCGA-LGG and TCGA-GBM. RTPCR was performed to detect LBX2-AS1 in surgery obtained normal brain and glioma. CCK-8 kit and Annexin V-FITC-PI Apoptosis Detection Kit were used to study the function of LBX2-AS1 on glioma proliferation and apoptosis. Bioinformatic analysis, RNA immunoprecipitation, RT-PCR, western blotting and dual luciferase reporter assay were carried out to investigate the target miRNA of LBX2-AS1. The discovered mechanism was validated by the rescue assay. Results: Following study of GTEx and TCGA data, LBX2-AS1 was significantly elevated in glioma compared with normal brain and in GBM compared with LGG. Higher expression of LBX2-AS1 was associated with poor prognosis of patients with glioma. Expression of LBX2-AS1 was positively correlated with pathology classification of glioma. Knockdown of LBX2-AS1 inhibited cell proliferation and induced cell apoptosis in glioma. LBX2-AS1 have complimentary binding site for tumor suppressor miR-491-5p and we showed that LBX2-AS1 sponged miR-491-5p to upregulate TRIM28 expression in glioma cells. TRIM28 overexpression attenuated the effect of LBX2-AS1 knockdown on glioma cells. Conclusions: In conclusion, LBX2-AS1 was an increased lncRNA in glioma. Mechanistically, LBX2-AS1 promoted glioma cell proliferation and resistance to cell apoptosis via sponging miR-491-5p. Keywords: LBX2-AS1, miR-491-5p, Cell proliferation, Cell apoptosis, Glioma, TRIM28 Background Gliomas are the most common primary cancer types derived from the neural ectoderm, accounting for approximately half of all brain malignancy [1]. Gliomas are histologic classified into several groups including grade II oligodendrogliomas and astrocytomas, and *Correspondence: [email protected] 1 Department of Neurosurgery, China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin, China Full list of author information is available at the end of the article
grade III anaplastic oligodendrogliomas, anaplastic astrocytomas, anaplastic oligoastrocytomas, anaplastic ependymomas, and grade IV glioblastomas (GBM) according to World Health Organization (WHO) classification [2, 3]. Overall, gliomas are lethal cancer type and patients with high grade glioma (GBM) have a median overall survival less than one year [4]. The worse prognosis of patients with glio
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