Rice OVATE family protein 6 regulates leaf angle by modulating secondary cell wall biosynthesis
- PDF / 7,774,282 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 88 Downloads / 170 Views
Rice OVATE family protein 6 regulates leaf angle by modulating secondary cell wall biosynthesis Xiaoxuan Sun1,2 · Yamei Ma1,2 · Chao Yang1,2 · Jianxiong Li1,2,3 Received: 23 September 2019 / Accepted: 20 July 2020 © Springer Nature B.V. 2020
Abstract Secondary cell wall not only provides rigidity and mechanical resistance to plants, but also has a large impact on plant growth and adaptation to environments. Biosynthesis of secondary cell wall is regulated by a complicated signaling transduction network; however, it is still unclear how the transcriptional regulation of secondary cell wall biosynthesis works at the molecular level. Here, we report in rice that OVATE family proteins 6 (OsOFP6) is a positive regulator in modulating expression of the genes related to biosynthesis of the secondary cell wall. Transgenic plants with knock-down of OsOFP6 by RNA interference showed increased leaf angle, which resulted from the thinner secondary cell wall with reduced amounts of cellulose and lignin, whilst overexpression of OsOFP6 in rice led to the thinker secondary cell wall with increased lignin content. Protein-protein interaction analysis revealed that OsOFP6 interacts with Oryza sativa homeobox 15 (OSH15), a class I KNOX protein. The interaction of OsOFP6 and OSH15 enhanced the transcriptional activity of OSH15 which binds to the promoter of OsIRX9 (Oryza sativa IRREGULAR XYLEM 9). Taken together, our study provides insights into the function of OsOFP6 in regulating leaf angle and the control of biosynthesis of secondary cell wall. Keywords Leaf angle · Mechanical strength · OVATE protein · Rice · Secondary cell wall · Xylan
Introduction OVATE family proteins (OFPs), a plant-specific family of regulatory proteins, were found to mediate multiple aspects of plant growth and development (Ku et al. 1999; Hackbusch et al. 2005; Ma et al. 2017; Yang et al. 2018). OFPs contain a conserved 70 amino acid C-terminal domain, Electronic supplementary material The online version of this article (doi:https://doi.org/10.1007/s11103-020-01039-2) contains supplementary material, which is available to authorized users. * Jianxiong Li [email protected] 1
Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Guangdong Provincial Key Laboratory of Applied Botany, Botanical Garden, Chinese Academy of Sciences, Guangzhou, South China 510650, China
2
University of Chinese Academy of Sciences, Beijing 100049, China
3
Core Botanical Gardens, Chinese Academy of Sciences, Guangzhou 510650, China
which was designated as the OVATE domain. The first OFP gene, OVATE, was characterized in tomato, and an occurring premature stop codon in it resulted in the transition of tomato fruit from round- to pear-shaped (Liu et al. 2002). Subsequently, OFP genes have been characterized in other species including Arabidopsis (AtOFPs) and rice (OsOFPs). Sequence analysis showed that there are 19 OFP genes in the Arabidopsis genome (Liu et al. 2014), and 31 genes encoding full-length OFPs in the rice genome
Data Loading...
