RNA Interference Techniques
Non-coding, inhibitory microRNAs have emerged as important modulators of cellular gene expression, through a process called RNA interference (RNAi). To date, hundreds of conserved and species-specific microRNAs have been identified in organisms rang
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Series Editor Wolfgang Walz University of Saskatchewan Saskatoon, SK, Canada
For further volumes: http://www.springer.com/7657
RNA Interference Techniques Edited by
Scott Q. Harper Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital, Columbus, OH, USA; Department of Pediatrics and Molecular, Cellular, and Developmental Biology Graduate Program, The Ohio State University College of Medicine, Columbus, OH, USA
Editor Scott Q. Harper Center for Gene Therapy The Research Institute at Nationwide Children’s Hospital Columbus, OH, USA and Department of Pediatrics and Molecular, Cellular and Developmental Biology Graduate Program The Ohio State University College of Medicine Columbus, OH, USA [email protected]
ISSN 0893-2336 e-ISSN 1940-6045 ISBN 978-1-61779-113-0 e-ISBN 978-1-61779-114-7 DOI 10.1007/978-1-61779-114-7 Springer New York Heidelberg London Dordrecht Library of Congress Control Number: 2011926455 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Series Preface Under the guidance of its founders Alan Boulton and Glen Baker, the Neuromethods series by Humana Press has been very successful since the first volume appeared in 1985. In about 17 years, 37 volumes have been published. In 2006, Springer Science + Business Media made a renewed commitment to this series. The new program will focus on methods that are either unique to the nervous system and excitable cells, or need special consideration to be applied to the neurosciences. The program will strike a balance between recent and exciting developments such as those concerning new animal models of disease, imaging, in vivo methods, and more established techniques, which include immunocytochemistry and electrophysiological technologies. New trainees in neurosciences still need a sound footing in these older methods in order to apply a critical approach to their results. Careful application of methods is probably the most important step in the process of scientific inquiry. In the past, new methodologies led the way in developing new disciplines in the biological and medical sciences. For example, physiology emerged out of anatomy in the nineteenth century by harnessing new me
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