Robust and Self-healable Antibiofilm Multilayer Coatings
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ARTICLE
POLYMER SCIENCE
https://doi.org/10.1007/s10118-021-2513-3 Chinese J. Polym. Sci.
Robust and Self-healable Antibiofilm Multilayer Coatings Chao Zhoua,†*, Jun-Tao Zhoua,†, Cheng-Ju Shenga, Dicky Pranantyob, Yan Pana, and Xiao-Jia Huanga a Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou 213164, China b Department of Chemical and Biomolecular Engineering, National University of Singapore, 117585 Singapore
Electronic Supplementary Information Abstract The infection induced by implantation of biomedical materials may result from the biofilm formation after bacteria attachment. Hence, the antibiofilm surface coating represents a novel technique to improve the antibacterial activity of biomedical materials. The traditional antibiofilm surface coatings exhibited some disadvantages and provided a limited service life. In this work, we used polyethyleneimine grafted 3maleimidopropionic acid (PEIM) and poly(acrylic acid) grafted 2-furfurylamine (PAAF) to achieve robust and self-healable crosslinked multilayer coatings, employing Layer-by-Layer (LbL) self-assembly technique and Diels-Alder reaction. Then, thiol-terminated poly((3-acrylamidopropyl) trimethylammonium chloride) (PAMPTMA-SH) was grafted onto the crosslinked multilayer coating by thiol-ene click reaction to form a novel multilayer coating (PEIM/PAAF)10-PAMPTMA. We found that this coating showed robust and self-healable activity, and significantly inhibited the bacterial growth and biofilm formation after infection with Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) by in vitro and in vivo assays for 120 h. In addition, the multilayer coating did not induce significant hemolysis or affect the cell viability of red blood cells. In vivo studies also showed that (PEIM/PAAF)10-PAMPTMA coating efficiently blocked the infiltration of inflammatory cells and gene expression in the mouse skin challenged with E. coli or S. aureus. Taken together, these results showed that the prepared multilayer coating exhibited strong antibiofilm activity and provided a new strategy for the application of highly efficient antibiofilm surface coating of biomedical materials. Keywords Layer-by-Layer assembly; Diels-Alder reaction; Self-healable; Antibiofilm coating; Thiol-ene click reaction Citation: Zhou, C.; Zhou, J. T.; Sheng, C. J.; Pranantyo, D.; Pan, Y.; Huang, X. J. Robust and self-healable antibiofilm multilayer coatings. Chinese J. Polym. Sci. https://doi.org/10.1007/s10118-021-2513-3
INTRODUCTION Microbial adhesion onto device/implant surfaces and formation of bacterial biofilms associated infections have become one of the challenging threats to biomedical devices in hospitals and implants in patients.[1−3] Therefore, many scientists developed various methods for preparing antibacterial or antifouling surface coatings such as exclusion steric repulsion,[4,5] electrostatic repulsion,[6,7] low surface energy,[8,9] biocide releasing,[10−13] and contact-active antimicrobial.[14−18] However, the occurrenc
