Role of porogenic solvent type on the performance of a monolithic imprinted column
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REVIEW
Role of porogenic solvent type on the performance of a monolithic imprinted column Belya A. Hermawan1 · Mutakin1 · Aliya N. Hasanah1 Received: 15 July 2020 / Accepted: 16 October 2020 © Institute of Chemistry, Slovak Academy of Sciences 2020
Abstract Due to many advantages, the use of imprinted monolith columns has become the choice to replace conventional particle columns. An imprinted monolith column has high permeability with minimal backpressure. Several factors can affect the performance of a column, such as the condition of synthesis, back pressure and the nature of the stationary phase. The latter feature relies on its constituent components, one of which lies in the pore structure. Another factor that influences the structure and morphology of pores is the porogenic solvent, where the selection of a porogen must be based on the solubility of all the polymer-forming components in it and must provide a sufficient pore size to facilitate the flow of the mobile phase. The use of porogenic solvents with different polarity levels and mixtures of more than one type of porogen can affect the pore structure of the formed polymers and, thus, also affect the performance of the resulting column. In this review, we will further discuss the role of porogenic solvent—polar or non-polar, single or mixed—on the performance of monolithic polymer columns as well as its applications. Keywords Porogenic solvent · Monolithic column · Imprinted polymer · Performance
Introduction HPLC is a refinement of liquid chromatography utilised for the separation of complex chemical and biological mixtures. HPLC first appeared in 1980 and continues to be used today. Indeed, it is considered to provide good specificity and precision (Siddiqui et al. 2017). HPLC is widely used for analysis, because it has several advantages, including rapid analysis and only requires small quantities of samples (Locatelli et al. 2014). In addition, HPLC is selective, easy to use, relatively good with regard to remanufacturing and easy to develop methods (Thota et al. 2015). HPLC has been applied for separation and analysis techniques such as safety and drug quality control (Gad and Levin 2010), biomarkers and chemical fingerprint analysis (Cook et al. 2015; He et al. 2015), drug abuse analysis (Bjørk et al. 2010) and various other pharmaceutical and biomedical sample analysis (Locatelli et al. 2014). Successful analysis using HPLC * Aliya N. Hasanah [email protected] 1
Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Padjadjaran University, Sumedang, Indonesia
relies on understanding and selecting proper chromatography systems. The chromatography system includes the type of detector, appropriate mobile phase selection and type of column (Gad and Levin 2010). The particle column for HPLC is usually a stationary phase, but it has markedly evolved recently as monolithic columns. These alternatives are useful given the shortage of conventional particle columns that are less selective and require a relat
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