Root-associated bacterial diversities of Oryza rufipogon and Oryza sativa and their influencing environmental factors
- PDF / 694,071 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 14 Downloads / 206 Views
ORIGINAL PAPER
Root‑associated bacterial diversities of Oryza rufipogon and Oryza sativa and their influencing environmental factors Lei Tian1,2 · Xue Zhou1 · Lina Ma1 · Shangqi Xu1,2 · Fahad Nasir1,3 · Chunjie Tian1
Received: 18 June 2016 / Revised: 6 November 2016 / Accepted: 7 December 2016 © Springer-Verlag Berlin Heidelberg 2016
Abstract Oryza rufipogon is the ancestor of human-cultivated Oryza sativa. However, little is known about the difference between the root-associated microorganisms of O. rufipogon and O. sativa. In this study, the root-associated bacteria of O. rufipogon, Leersia hexandra, and O. sativa from different latitudes in China were studied by DGGE analysis. Their bacterial community structures were compared by principal component analysis. The relationship between root-associated bacteria and soil properties was explored by canonical correspondence analysis. The relationships of glomalin-related soil protein (GRSP) content, soluble sugar content, proline content of the plant, and bacterial diversity indices of their root-associated microorganisms were also investigated. We found both broad-spectrum and host-specific bacteria, and the similarity, diversity and abundance indices of O. rufipogon and L. hexandra were higher than O. sativa root-associated bacteria. However, even living in the same habitat, O. rufipogon and L. hexandra selected different root-associated bacteria. Microbial composition was primarily correlated with available N, P,
Communicated by Erko Stackebrandt. Electronic supplementary material The online version of this article (doi:10.1007/s00203-016-1325-2) contains supplementary material, which is available to authorized users. * Chunjie Tian [email protected] 1
Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin, China
2
University of Chinese Academy of Sciences, Beijing, China
3
School of Life Sciences, Northeast Normal University, Changchun, Jilin, China
and K and the annual precipitation. We also found a positive correlation between the soluble sugar content of the plant and GRSP content of the root soil. The above results indicated that the community structure of root-associated bacteria differs between wild rice and cultivated rice. Human activity and the natural selection of the host plants shaped the differences, consistent with our hypothesis. Keywords Oryza rufipogon · Oryza sativa · DGGE · Root-associated bacteria · Environmental factors
Introduction Asian cultivated rice (Oryza sativa) was domesticated from wild common rice (Oryza rufipogon) by humans during the long history of human agriculture (Londo et al. 2006). Currently, O. sativa is the most important food resource in the world. China has the largest rice planting area and production worldwide and is the origin of Asian cultivated rice (Li et al. 2006). Cultivated rice lost many desirable traits, such as disease resistance, during the process of domestication from wild common rice. These lost desirab
Data Loading...
