Safety and efficacy of human amniotic membrane in primary pterygium surgery
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ORIGINAL PAPER
Safety and efficacy of human amniotic membrane in primary pterygium surgery Nagi Marsit . Nagmeden Gafud . Inass Kafou . Adel Mabrouk . Aml Alatiweel . Sedigh Abdalla . Lamia Sheghewi
Received: 12 February 2016 / Accepted: 7 April 2016 Ó Springer Science+Business Media Dordrecht 2016
Abstract Grafts made from human amniotic membrane are used to prevent recurrence of pterygium after excision. The success of the procedure can be affected by the quality of preparation and preservation of the grafts. We prospectively evaluated the safety and efficacy of cryopreserved amniotic membrane prepared at the research tissue bank of the Biotechnology Research Center in Tripoli, Libya, and used as adjunct therapy in primary pterygium excision. Twenty-six patients (15 males and 11 females) aged 21–78 years and indicated for primary pterygium excision were transplanted at the Tripoli Eye Hospital with the amniotic membrane grafts. Sixteen patients (62 %) were available for all three follow-up visits scheduled at 1, 3 and 6 months post-surgery. By the third visit, two patients (12.5 %) developed granuloma and three (18.8 %) had pterygium recurrence. The grafts were used after cryopreservation for B180 days or [180 days, but statistical analysis showed that the complications were not associated with the length of storage. Moreover, the high rate of complications in this study was not caused by use of cryopreserved AM. N. Marsit (&) A. Mabrouk S. Abdalla Tissue Banking Research Group, Biotechnology Research Centre (BTRC), Tweisha, Libyan Authority for Research, Sciences and Technology, P. O. Box 30313, Tripoli, Libya e-mail: [email protected] N. Gafud I. Kafou A. Alatiweel L. Sheghewi Tripoli Eye Hospital, Tripoli, Libya
In conclusion, locally produced cryopreserved AM is safe as an adjunct therapy for treatment of primary pterygium excision. Keywords Amniotic membrane Transplantation Graft Cryopreservation Pterygium Eye surgery
Introduction Pterygium is a common benign growth of the conjunctiva on the sclera, which impairs vision. Its pathogenesis is linked to environmental factors such as frequent exposure to ultraviolet light in hot, dry, windy and dusty areas and smoky environments (Koranyi et al. 2004). The tumor suppressor gene p53 has been implicated in the pathogenesis of pterygium, as well as several other genes involved in DNA repair, cell proliferation and migration, and angiogenesis, indicating the interaction of genetic and environmental factors (Liu et al. 2013). Pterygium is commonly treated by surgical excision, and recurrence is the most common complication following surgery. There are different surgical options: bare sclera, conjunctival and limbal autografts, mitomycin C, lamellar keratoplasty and amniotic membrane (AM) transplantation (Hirst 2003). AM is the innermost layer lining the foetal sac in the uterus. It is widely used as a wound healing material in many disciplines, including ophthalmology. AM is
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Cell Tissue Bank
processed using different protocols: fres
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