Salmonella typhimurium detector based on the intrinsic peroxidase-like activity and photothermal effect of MoS 2
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ORIGINAL PAPER
Salmonella typhimurium detector based on the intrinsic peroxidase-like activity and photothermal effect of MoS2 Shuyuan Du 1 & Zhang Lu 1 & Luxiang Gao 1 & Yuanyuan Ge 1 & Xiaoyu Xu 1 & Hongyan Zhang 1 Received: 1 July 2020 / Accepted: 10 October 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A multimode dot-filtration immunoassay (MDFIA) was established for rapid and accurate detection of the target (Salmonella typhimurium), which was based on the intrinsic color, peroxidase-like activity and photothermal effect of molybdenum disulfide (MoS2). Obviously, multimode detection can improve detection accuracy compared to the direct visual detection in test strips. A thermal imaging camera was used as detector to record the temperature change (ΔT) of MoS2 and establish the standard curve of ΔT and the concentration of Salmonella typhimurium to realize quantitative determination. The main parameters that affect the analytical performance of MDFIA were optimized. Under the optimal experimental conditions, the limit of detection (LOD) of photothermal detection reached 102 CFU mL−1 and was one order of magnitude lower than the limit of direct visual detection and catalytic color development detection (103 CFU mL−1). The accuracy and analytical sensitivity were enhanced by intrinsic peroxidase-like activity and the huge photothermal effect of MoS2. Moreover, this method exhibited high selectivity, good repeatability, and acceptable stability and the entire process was simple to be accomplished in 30 min, which generally meets the need of rapid detection. The successful implementation in real samples with the recovery being between 99.5 and 119.2% showed that it could be used as a promising quality control strategy for detection of other foodborne pathogens. Keywords MoS2 . Peroxidase-like activity . Photothermal effect . MDFIA . Salmonella typhimurium
Introduction Salmonella typhimurium is responsible for foodborne illness of human and animals every year, which not only poses a threat to human health but also causes economic cost to society. Salmonella typhimurium is widely distributed in the environment [1] and transmits through Salmonella typhimurium–contaminated foods such as eggs [2], milk [3], fruits [4], and poultry [5]. Many detection methods of Salmonella typhimurium have been developed, including traditional Shuyuan Du and Zhang Lu contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04600-4) contains supplementary material, which is available to authorized users. * Hongyan Zhang [email protected] 1
Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Science, Shandong Normal University, Jinan 250014, People’s Republic of China
microbiological culture method [6], polymerase chain reaction [7], loop-mediated isothermal amplification test [8], e
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