Screening of Acetate-Tolerant Yeast and Its Effect on Controlling Bacterial Contamination During Ethanol Production from
- PDF / 776,974 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 37 Downloads / 178 Views
RESEARCH ARTICLE
Screening of Acetate-Tolerant Yeast and Its Effect on Controlling Bacterial Contamination During Ethanol Production from Sugarcane Molasses Pramuan Saithong1,2 • Wirat Vanichsriratana1 • Sumallika Morakul1
Received: 25 February 2020 / Accepted: 21 September 2020 Society for Sugar Research & Promotion 2020
Abstract Growth and ethanol fermentation ability of the acetate-tolerant yeast Saccharomyces cerevisiae KU 002-3 strain were investigated in acetate-addition medium. Under simulated industrial conditions, acetate did not affect on sugar consumption and ethanol production of KU 002-3. Moreover, KU 002-3 strain demonstrated a higher capability for ethanol production than other commercial strains. Co-fermentation was conducted with Lactobacillus fermentum IFRPD 2021, the major contaminating bacteria, during the ethanol production at 0.8% (v/v) acetate (130 mM of the undissociated form of acetate). Under these conditions, growth of L. fermentum IFRPD 2021 was inhibited but the growth and ethanol production ability of S. cerevisiae KU 002-3 strain were not significantly affected. Data indicated the possibility of producing ethanol under high acetate accumulation and reducing Lactobacillus sp. in molasses medium or acetate-containing condition. Producing ethanol under high acetate accumulation and lower the contamination of Lactobacillus sp. in a molasses medium can be useful at industrial scale to produce ethanol with S. cerevisiae KU 002-3 under non-sterilized conditions.
& Sumallika Morakul [email protected] 1
Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, 50 Ngamwongwan Rd. Chatuchak, Bangkok 10900, Thailand
2
Department of Applied Microbiology, Institute of Food Research and Product Development, Kasetsart University, 50 Ngamwongwan Rd. Chatuchak, Bangkok 10900, Thailand
Keywords Ethanol Acetate-tolerant yeast Bacterial contamination Lactobacillus sp. Sugarcane molasses
Introduction Over the past 10 years, the ethanol industry has continued to grow steadily, supported by a global shift toward a green economy. Thailand is one of the largest bioethanol producing and exporting country as the sixth largest ethanol producer, with a production capacity of around 5.2 million liters/day and this is expected to increase to 7.2 million liters/day by 2024 (OECD/Food and Agriculture Organization of the United Nations 2015). Supportive government policies are key growth drivers for the Thai ethanol industry. The ‘‘Alternative Energy Development Plan (AEDP 2015)’’ was released as a 20-year plan by the Ministry of Energy. The goal is to increase current production to 11.2 million liters/day by 2036. If everything goes as planned, the Thai ethanol industry will grow at a rate of 7% per year and demand for sugarcane molasses, and the main raw material for ethanol production will increase to 182 million tons/year in 10 years’ time to achieve the goals set by AEDP 2015 (Teerayut 2015). Thailand already has 26 ethanol plants, and 57.13% of the country’s molasses is th
Data Loading...
