Seasonal Variations of Picoeukaryote Community Structure in Zhoushan Fishing Ground, East China Sea
- PDF / 750,309 Bytes
- 9 Pages / 595.22 x 842 pts (A4) Page_size
- 62 Downloads / 158 Views
Seasonal Variations of Picoeukaryote Community Structure in Zhoushan Fishing Ground, East China Sea YANG Mingyu1), #, LIU Qiang1), 2), #, MI Chunrong3), and ZHAO Shujiang1), * 1) National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan 316022, China 2) Chinese Research Academy of Environmental Sciences, Institute of Water Environment Science, Beijing 100020, China 3) Institute of Zoology, Chinese Academy of Sciences, Beijing 100000, China (Received September 4, 2019; revised June 15, 2020; accepted July 28, 2020) © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2020 Abstract Picoeukaryotes (< 2–3 μm) are major oceanic primary producers and play a crucial role for the functioning of marine ecosystems. However, the community structure of such communities remains poorly understood, especially in the East China Sea (ECS). We investigated the seasonal variations of abundance and diversity of picoeukaryotes, and recorded environmental variables, along a transect in Zhoushan Fishing Ground. High-throughput sequencing was used for sequencing the V4–V5 variable region within the 18S rRNA gene to analyze genetic diversity and relative abundance of picoeukaryotes. A total of 40 phyla, 68 classes, 99 orders, 126 families, and 140 genera were observed. The estimated richness and diversity indices were both higher in each season. The composition and dominant picoeukaryotes changed significantly at the class level with the seasons alternating. Basidiomycota, Ciliophora, Ascomycota and Cryptomonadales were observed throughout the year. The Linear Discriminant Analysis (LDA) revealed the predominant discriminant taxa of four seasonal groups. Redundancy analysis (RDA) showed that NO2− and salinity played very important roles to picoeukaryotes for all the spring samples and DS5 sample in winter, PO43− and pH affected mostly picoeukaryotes for winter samples and autumn samples. Key words versity
picoeukaryotes; Zhoushan Fishing Ground; high-throughput sequencing; 18S rRNA gene; community structure; di-
1 Introduction Picoeukaryotes (Peuk), defined operationally as cells no more than 2–3 μm in diameter, are the most abundance of eukaryotic species on the earth (Li 1994; Jardillier et al., 2010). They appear in the global marine environments and are considered as basic components of marine ecosystems in the oceans in the light of their significant biomass and high primary productivity (Falkowski et al., 2008; Massana et al., 2011; Caron et al., 2012). Picoeukaryotes, as a critical member of the micro-food web, mainly include picophytoplankton and various protozoa, organically linked the micro-food web and the classic food web by feeding on ciliates (Christaki et al., 2009). Due to its extremely small cell size and the lack of significant morphological features, it was only since the introduction of flow cytometry into the field of microbial ecology in the 1980s that the research on picoeukaryotes #
The two authors contributed equally to this work. * Correspo
Data Loading...
