Selective determination of catecholamine in the presence of ascorbic acid or uric acid on the membrane of silver nanopar
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ORIGINAL PAPER
Selective determination of catecholamine in the presence of ascorbic acid or uric acid on the membrane of silver nanoparticles/poly L-phenylalanine Mei Qin & Kewei Cao & Xueliang Wang & Huanhuan Xu & Zhangyu Yu
Received: 31 March 2013 / Revised: 16 June 2013 / Accepted: 18 June 2013 # Springer-Verlag Berlin Heidelberg 2013
Abstract The silver ions and L-phenylalanine were codeposited and formed a hybrid membrane on the surface of glassy carbon electrode by cyclic voltammetry. The membrane had good properties for catalyzing the redox of catecholamine neurotransmitters, including epinephrine (EP), norepinephrine (NE), and dopamine (DA). The electrochemical behaviors of these neurotransmitters were studied on this modified electrode. and therefore, an assay for each of them is set up and the detection limits for EP, NE, and DA are 7.2×10−9, 6.4×10−9, and 8.5×10−9 mol L−1, respectively. The proposed method can effectively eliminate the interference of the ascorbic acid and uric acid. The conditions which influenced the analyses were optimized. Using this method to determine the content of EP, NE, and DA in injections, the results were satisfactory. Keywords Catecholamine . Silver . Poly L-phenylalanine . Ascorbic acid . Uric acid
Introduction Catecholamine, generally including epinephrine (EP), norepinephrine (NE), and dopamine (DA), is a kind of important Electronic supplementary material The online version of this article (doi:10.1007/s11581-013-0946-6) contains supplementary material, which is available to authorized users. M. Qin : K. Cao : H. Xu : Z. Yu College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu 273165, People’s Republic of China X. Wang : Z. Yu (*) Department of Chemistry and Chemical Engineering, Heze University, Heze 274015, People’s Republic of China e-mail: [email protected]
neurotransmitter containing catechol and amidocyanogen in their molecule structure. Its change of concentration level in biological fluid may induce many diseases, such as Parkinson’s disease, schizophrenia, and HIV infection [1–3]. For this reason, catecholamine drugs are usually used to treat anaphylactic shock, bronchial asthma, and organic heart disease [4–6]. Therefore, developing a simple and fast method for determination of catecholamine has been a focal subject in bioscience, biotechnology, and medicinal chemistry, especially in neurochemistry [7], and many methods, such as chromatographic methods [8, 9], spectroscopic methods [10, 11], chemiluminescence [12, 13], capillary electrophoresis [14, 15] and flow injection analysis [16], and various electrochemical methods [17–22] have been developed. Among of these methods, electrochemical methods are simple and sensitive. However, using electrochemical method for determination of the catecholamine, two important problems must be circumvented. The one is that EP, NE, and DA at conventional bare electrodes yield poor electrochemical response; another problem is that EP, NE, and DA usually coexist in natural samples with ascorbic and uri
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