Validated Electrochemical Method for Simultaneous Resolution of Tyrosine, Uric Acid, and Ascorbic Acid at Polymer Modifi
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alidated Electrochemical Method for Simultaneous Resolution of Tyrosine, Uric Acid, and Ascorbic Acid at Polymer Modified Nano-Composite Paste Electrode Chenthattil Rarila, Jamballi G. Manjunathaa, *, Doddarasinakere K. Ravishankarb, Santosh Fattepurc, Gurumallappa Siddarajud, and Lingappa Nanjundaswamye a
Department of Chemistry, FMKMC College, Madikeri, Mangalore University Constituent College, Karnataka, 571201 India bDepartment of Chemistry, Sri Mahadeshwara Goverment First Grade College, Kollegal, Chamarajanagar, Karnataka, 571440 India cSchool of Pharmacy, Management and Science University, 40100 Malaysia dDepartment of Chemistry, JSS College for women Chamarajanagar, Karnataka, 571313 India eUGC – Human Resource Development Centre, University of Mysore, Manasagangotri, Karnataka, Mysore, 570006 India *e-mail: [email protected] Received October 28, 2019; revised November 11, 2019; accepted November 18, 2019
Abstract—In this work, an electrochemical sensor based on a poly threonine modified graphite-carbon nanotube paste electrode was developed for the investigation of tyrosine. The modification of the electrode was characterized by field emission scanning electron microscopy, cyclic voltammetry, and differential pulse voltammetry. The modified electrode shows many advantages such as simple preparation, good sensitivity, short response time, good stability and reproducibility. The developed electrode was highly selective because of the determination of tyrosine in the presence of the ascorbic acid and the uric acid. Under optimal conditions; the cyclic voltammetry provides a linear response with the concentration range from 2 × 10–6 to 2.5 × 10–5 M and 3 × 10–5 to 1.2 × 10–4 M with the limit of detection and limit of quantification values of 2.9 × 10–7 and 9.6 × 10–7 M. The developed sensor was employed for tyrosine detection in pharmaceutical sample, recoveries obtained were in a range of 99.0 to 102.80%. Keywords: tyrosine, carbon nanotube, cyclic voltammetry, graphite powder, biosensor DOI: 10.3103/S1068375520040134
INTRODUCTION Tyrosine (Tyr) is an essential aromatic amino acid precursor for thyroxine, dopamine, adrenalin, and noradrenalin [1]. As reported elsewhere, high Tyr containing foods such as cheese, eggs, dairy, and beans could improve people’s memory when under stress [2]. Hypochondrium, depression, and some other psychological diseases can be results of the deficiency of Tyr [3]. Therefore, it becomes a paramount task to develop an efficient analytical method for the resolution of Tyr in nutriment samples. The uric acid (UA) is a major nitrogenous compound in urine. The concentration of UA is around 2 mM in urine and 120 to 450 mM in the blood of healthy persons. Normally, healthy human beings excrete 400 to 700 mg urine per day. The abnormal UA levels reveal such problems as gout, hyperuricemia, or Lessch–Nyhan syndrome. Ascorbic acid (AA) is a water-soluble vitamin that is found in biological systems and foodstuffs; it acts as strong antioxidant [4]. The surplus of amino acids can lead to g
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