Sequence analysis and spatiotemporal developmental distribution of the Cat-1-type transporter slc7a1a in zebrafish ( Dan
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Sequence analysis and spatiotemporal developmental distribution of the Cat-1-type transporter slc7a1a in zebrafish (Danio rerio) Ståle Ellingsen & Shailesh Narawane & Anders Fjose & Tiziano Verri & Ivar Rønnestad
Received: 22 May 2020 / Accepted: 1 September 2020 # The Author(s) 2020
Abstract Cationic amino acid transporter 1 (Cat-1 alias Slc7a1) is a Na+-independent carrier system involved in transport and absorption of the cationic amino acids lysine, arginine, histidine, and ornithine and has also been shown to be indispensable in a large variety of biological processes. Starting from isolated full-length zebrafish (Danio rerio) cDNA for slc7a1a, we performed comparative and phylogenetic sequence analysis, investigated the conservation of the gene during vertebrate evolution, and defined tissue expression during zebrafish development. Whole mount in situ hybridization first detected slc7a1a transcripts in somites,
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10695-020-00873-x) contains supplementary material, which is available to authorized users. S. Ellingsen : S. Narawane : A. Fjose Department of Molecular Biology, University of Bergen, Postbox 7803, NO-5020 Bergen, Norway I. Rønnestad (*) Department of Biological Sciences, University of Bergen, Postbox 7803, NO-5020 Bergen, Norway e-mail: [email protected] T. Verri Department of Biological and Environmental Sciences and Technologies, University of Salento, via Prov.le Lecce-Monteroni, I-73100 Lecce, Italy
Present Address: S. Ellingsen Department of Biological Sciences, University of Bergen, Postbox 7803, NO-5020 Bergen, Norway
eyes, and brain at 14 h post-fertilization (hpf) with additional expression in the distal nephron at 24 hpf and in branchial arches at 3 days post-fertilization (dpf), with significant increase by 5 dpf. Taken together, the expression analysis of the zebrafish Cat-1 system gene slc7a1a suggests a functional role(s) during the early development of the central nervous system, muscle, gills, and kidney.
Keywords Cationic amino acid transport(ers) . slc7a1a . slc7a1b . Spatiotemporal expression . Zebrafish
Introduction The cationic amino acid transporter 1 (Cat-1), a member of the solute carrier (SLC) 7 family of proteins, also referred to as solute carrier family 7 member 1 (Slc7a1), is a Na+-independent transporter of cationic amino acids. In mammals, the Cat protein group consists of Cat-1, Cat-2a, Cat-2b, and Cat-3, all exhibiting a nearly identical substrate specificity for cationic L-amino acids (Deves et al. 1998; Closs et al. 2004; Closs et al. 2006). Cat-4 is acknowledged to have no affinity for cationic, neutral, or acidic amino acids and is referred to as an orphan transporter, similarly to the Cat protein referred to as Slc7a14 (Closs et al. 2006). So far, very little information is available on the molecular and functional characterizations of these transporters in teleost fish (Gu et al. 2014).
Fish Physiol Biochem
Cat proteins are predicted to have 14 transmembr
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