Silica gel dissication of amniotic membrane with related epithelium cells for ocular surface reconstruction
- PDF / 74,167 Bytes
- 5 Pages / 595 x 842 pts (A4) Page_size
- 94 Downloads / 183 Views
271
-1
Silica gel dissication of amniotic membrane with related epithelium cells for ocular surface reconstruction E.S. Miljudin1,*, A.V. Zolotaryov1, L.T. Volova2 and U.M. Ahmerova1 1
Eye Tissue Bank, Ophthalmology Hospital, Novo-Sadovaja str. 158, 443068 Samara, Russia; 2Samara State Medical University; *Author for correspondence (e-mail: [email protected])
Received 11 March 2004; accepted in revised form 9 July 2004
Key words: Amniotic membrane, Cornea, Drying over silica gel, Epithelium cells, Eyeball burns
Abstract Cornea reparative regeneration when in various pathological states needs creating certain conditions to intensify the potential of regional stem cells mitotic activity. Aims of the Research. To find out the degree of the epithelium AM preservation after preliminary processing and conservation by means of dehydration over silica gel with further sterilization; to study the effectiveness of clinical treatment of AM conserved in the surroundings with vital ability epithelium and AM dried over silica gel. Materials and methods. There was carried out an investigation of 18 samples of native amnion treated with antibiotics and 18 total surface amnion samples conserved by drying over silica gel and then sterilized by gamma rays. Clinical experiments were carried out on patients with severe chemical and thermal burns – 18 people (21 eyes). After the burn trauma all the patients underwent the standard procedure of necrectomy, the covering of the eyeball with amniotic membrane dried over silica gel. Conclusion. The drying out of the amniotic membrane over silica gel on frames without being fixed on nitrocellulose paper makes the process of the amniotic membrane conservation simpler and makes it possible to preserve its unique biological qualities. The effectiveness of the regeneration of epithelium tissue of the eyeball surface with amniotic membrane dried over silica gel without the vital capacity cells of the epithelium layer is analogous to the regeneration of epithelium cells with amniotic membrane with vital capacity cells. With eye burns AM coverage hinders the formation of rough conjunctiva cicatrix, provides a favorable out-of-cell matrix substrate for epithelium migration and leads to quicker regeneration of one’s own epithelium, makes further visual rehabilitation simpler.
Introduction Cornea reparative regeneration, when in various pathological states, needs creating certain conditions to intensify the potential of regional stem cells mitotic activity. The search for materials to create these conditions led to a rediscovery of amniotic membrane. The anatomic structure, peculiarities of tissue and the functions of the amnion determine the qualities that attract research-workers and clinic specialists and make
them use amniotic membrane when treating various pathological states. Used for the making of the transplant the out-of-placenta zone of the amnion is carpeted v/with cubic epithelium. Amnion epithelium produces glycoproteins, interlinings, growth factors (Champliaud et al. 1996). These substances
Data Loading...
