Silicon Microchannel Array as a Basis of Biosensor Device
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0915-R03-06
Silicon Microchannel Array as a Basis of Biosensor Device Alexander Lomzov1, Natalia Vandysheva2, Alexander Bublikov2, Dmitry Pyshnyi 1, and Sergei Romanov2 1 Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of RAS, Lavrentiev Ave.8, Novosibirsk, 630090, Russian Federation 2 Institute of Semiconductor Physics, Siberian Branch of RAS, Lavrentiev Ave.13, Novosibirsk, 630090, Russian Federation
ABSTRACT This work shows the possibilities of using the silicon-based microchannel array (Si-MCA) as a 3D-solid support of DNA probes and studying of application of optical methods for DNA hybridization direct analysis. It was obtained that amount of immobilized oligodeoxyribonucleotides on the Si-MCA surface is enhanced with lighting of Si-MCA microchannel. The sample of 160 µm thickness succeeded in immobilizing oligonucleotides 40 times as much as the standard glass slides having the same external surface. The application of Si-MCA as a carrier of DNA allows doing direct analysis of nucleic acids in infrared region by the FTIR method that essentially simplifies creation of a biosensor based on the Si-MCA. It is indicated that using of the Si-MCA as a diffraction grating in visible and UV spectra allows simplifying the spectral analysis of biochemical reaction. It was success confidently to distinguish the mediums differing on the refractive index by ∆n=0.006. INTRODUCTION Up-to-date diagnostics of different diseases are based on DNA analysis using a method of molecular hybridization [1, 2]. The method involves detection of complementary complexes formed by the oligonucleotide probes and DNA targets in a highly parallel fashion [2, 3]. So far, most systems, developed for diagnostics and molecular medicine, were based on a twodimensional microarray format. More recently, to enhance the hybridization reactions as well as to provide increased sensitivity and dynamical range, higher throughput and shorter assay times, three-dimensional microchannel arrays have been developed [3-6]. Here, the effective surface area available for probe immobilization is increased by expansion into the third dimension, therefore a higher density of probes can be achieved as compared to a planar substrate with the same lateral dimensions [3]. To present day, microchannel glass substrates and anodic porous alumina membranes have been used as three-dimensional arrays for multiplexed nucleic acid fluorescence hybridization assays [3-6]. In this paper analytical possibility of a newly emerged silicon-based microchannel array (Si-MCA) for DNA analysis using optical techniques have been studied. When compared to glass or porous alumina arrays, the Si-MCA provides a much wider choice of its structural parameters, functional materials and can be easily integrated with different Nano- and Microelectro-mechanical systems. EXPERIMENT A silicon microchannel array is a two-dimensional arrangement of square-mesh microchannels with vertical walls with a length up to hundreds of micrometers and lateral
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