Simultaneous Determination of Atractylenolide II and III in Rhizoma Atractylodes Macrocephalae and Chinese Medicinal Pre
This present paper describes the development of a reverse-phase high-performance liquid chromatography (RP-HPLC) coupled with UV spectrometry for simultaneous determination of atractylenolide II and III in traditional Chinese medicines and Chinese medicin
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Simultaneous Determination of Atractylenolide II and III in Rhizoma Atractylodes Macrocephalae and Chinese Medicinal Preparation by Reverse-Phase High-Performance Liquid Chromatography Xiao-hong Sun and Jian Ge Abstract This present paper describes the development of a reverse-phase high-performance liquid chromatography (RP-HPLC) coupled with UV spectrometry for simultaneous determination of atractylenolide II and III in traditional Chinese medicines and Chinese medicinal preparation. The standard of atractylenolide II and III was isolated from Rhizoma Atractylodes Macrocephalae. From a variety of solvents tested, methanol was found to be the best solvent for simultaneously extracting atractylenolide II and III from Rhizoma Atractylodes Macrocephalae and Chinese medicinal preparation samples. RP-HPLC analysis of the extracts was performed on an analytical column (SHIMADZU ODS, 150 9 4.6 mm; i.d., 5 lm) equipped with a security guard pre-column system. The results showed that there was a good linearity over the range 0.5–50.0 lg/ml (r [ 0.99). The recoveries were more than 95.0 % in Rhizoma Atractylodes Macrocephalae, and the intra- and inter-day coefficients of variation were less than 5.0 % in all cases. The lower limit of quantification (LLOQ) was 0.5 lg/ml. The RP-HPLC method was readily applied to simultaneously quantitate atractylenolide II and III in Rhizoma Atractylodes Macrocephalae and Chinese medicinal preparation (Shenlingbaizhu granule). Keywords Atractylenolide II atractylodes macrocephalae
Atractylenolide III
RP-HPLC
Rhizoma
X. Sun Shaoxing University Yuanpei College, Shaoxing 312000, China J. Ge (&) College of Life Science, China Jiliang University, #258 XueYuan Street, XiaSha Higher Education Zone, Hangzhou 310018, Zhejiang Province, People’s Republic of China e-mail: [email protected]
S. Li et al. (eds.), Frontier and Future Development of Information Technology 1283 in Medicine and Education, Lecture Notes in Electrical Engineering 269, DOI: 10.1007/978-94-007-7618-0_134, Springer Science+Business Media Dordrecht 2014
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X. Sun and J. Ge
134.1 Introduction Atractylenolide II and atractylenolide III, as marker substances in Rhizoma Atractylodes Macrocephalae, possess well-documented gastrointestinal inhibitory effects, anti-inflammatory and anticancer activity [1–3]. Rhizoma A. macrocephala is the dried rhizoma of Atractylodes macrocephala Koidz, which has been widely used in China as a herbal medicine, and reported as a nutrient for energy and for the treatment of dyspepsia and anorexia [4]. Rhizoma A. macrocephala was also contained in many Chinese medicinal prescriptions such as DangguiShaoyao-San (DSS). The essential oil has been isolated and is the major constituent of Rhizoma A. macrocephala, which has several pharmacological functions [5, 6]. In the procedures of screening and locating the effective compounds contained in DSS, Gu and his colleagues found that the volatile oil fraction extracted from DSS contributed most to the prevention and treatment of senile demen
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