Simultaneous determination of flavonoids in Oroxylum indicum by RP-HPLC
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Med Chem Res (2013) 22:2222–2227 DOI 10.1007/s00044-012-0214-8
ORIGINAL RESEARCH
Simultaneous determination of flavonoids in Oroxylum indicum by RP-HPLC Akhilesh K. Yadav • N. Manika • Guru D. Bagchi Madan M. Gupta
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Received: 13 October 2011 / Accepted: 24 August 2012 / Published online: 6 September 2012 Ó Springer Science+Business Media, LLC 2012
Abstract A reverse-phase liquid chromatographic method has been established for analysis of oroxylin A, chrysin, baicalein, and hispidulin in the roots of Oroxylum indicum (Bignoneaceae). Under the optimized conditions, wellresolved separation of pure compounds with significantly different Rt values was achieved on chromolith C18 column (100 mm 9 4.6 mm, 10 lm) using isocratic elution of acetonitrile and water (0.1 % trifluoroacetic acid); with acceptable validation results such as linearity, sensitivity, and recovery in terms of %RSD. The calibration curves were linear in the concentration range of 200–500 ng ml-1. Method was validated as per ICH guidelines. The developed reproducible and robust method may be applied for the quality assays of O. indicum and phytopreparations. Keywords HPLC
Oroxylum indicum Isolation Flavonoids
Introduction Oroxylum indicum Vent. (Syonakh) belongs to the family Bignoniaceae, its young shoots, flowers and unripe fruits are consumed as vegetable in northeastern Asia (Facciola, 1990). The tree is distributed in India, Ceylon, Malaysia, Cochin, China, Philippines, Thailand, and Indonesia A. K. Yadav M. M. Gupta (&) Analytical Chemistry Department, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP Campus, Lucknow 226 015, India e-mail: [email protected] N. Manika G. D. Bagchi Taxonomy and Pharmacognosy Department, Central Institute of Medicinal and Aromatic Plants, Lucknow 226015, India
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(Anonymous, 1972). Plant is an important herbal medicine in many Asian countries and is used in folk medicines as a cure of various diseases (Biswas and Ghosh, 1994). Root bark is used in fever, bronchitis, intestinal worms, leucoderma, asthma, inflammation, anal troubles, etc. The fruits and seeds are used as expectorant, purgative, and bitter tonic (Kirtikar and Basu, 1996). In Hindu medicine, the root bark, stem, and leaf are prescribed for snake bite, diarrhea, and dysentery (Ghani, 1998). Plant is reported to possess anti-inflammatory, analgesic, diuretic, gastroprotective, anti-arthritic, antimutagenic, antifungal, and antibacterial activities (Warrier et al., 2003; Zaveri and Jain, 2010). O. indicum is used as one of the important ingredients of well-known ayurvedic preparation ‘‘Dashmool’’. It is also used in other ayurvedic formulations such as amartarista, dantyadyarista, narayana taila, dhanawantara ghrita, brahma rasayan, and chyavanaprasa awalwha. It supplements traditional ayurvedic medicines to alleviate thirst, rheumatism, dysentery, anorexia, bronchitis, eruptive fevers, and dropsy (Anonymous, 1998). Phytochemically, plant contains flavonoids like baicalein, chrysin, oroxylin A, apigenin, tetuin, and s
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