Single-Cell Dosing and mRNA Sequencing of Suspension and Adherent Cells Using the PolarisTM System
Single-cell functional analysis provides a natural next step in the now widely adopted single-cell mRNA sequencing studies. Functional studies can be designed to study cellular context by using single-cell culture, perturbation, manipulation, or treatment
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Valentina Proserpio Editor
Single Cell Methods Sequencing and Proteomics
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Single Cell Methods Sequencing and Proteomics
Edited by
Valentina Proserpio Department of Life Sciences and System Biology, University of Turin, Italian Institute for Genomic Medicine, IIGM Turin, Torino, Italy
Editor Valentina Proserpio Department of Life Sciences and System Biology University of Turin, Italian Institute for Genomic Medicine, IIGM Turin Torino, Italy
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9239-3 ISBN 978-1-4939-9240-9 (eBook) https://doi.org/10.1007/978-1-4939-9240-9 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface From the first mRNA-Seq whole-transcriptome analysis in 2009, in less than 10 years, many new technologies and strategies have been rapidly developed in order to analyze the genome, transcriptome, and proteome of individual cells, scaling from few to hundreds of thousands of cells analyzed at a time. Since then, many new biological questions have opened, and many laboratories across the world have utilized single-cell omics for their research, with a parallel massive increase in the number of publications regarding single cells. Keeping up with such a rapidly evolving technology is not an easy task, and for someone that enters the “single-cell field” for the first time, this might look like a maze, a ju
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