Small grain and semi-dwarf 3, a WRKY transcription factor, negatively regulates plant height and grain size by stabilizi
- PDF / 10,574,157 Bytes
- 22 Pages / 595.276 x 790.866 pts Page_size
- 103 Downloads / 168 Views
Small grain and semi-dwarf 3, a WRKY transcription factor, negatively regulates plant height and grain size by stabilizing SLR1 expression in rice Jie Lan1 · Qibing Lin2 · Chunlei Zhou1 · Yakun Ren1 · Xi Liu1 · Rong Miao1 · Ruonan Jing1 · Changling Mou1 · Thanhliem Nguyen1,3 · Xingjie Zhu1 · Qian Wang1 · Xin Zhang2 · Xiuping Guo2 · Shijia Liu1 · Ling Jiang1 · Jianmin Wan1,2 Received: 15 February 2020 / Accepted: 6 August 2020 © Springer Nature B.V. 2020
Abstract Key message OsWRKY36 represses plant height and grain size by inhibiting gibberellin signaling. Abstract Plant height and grain size are important agronomic traits affecting yield in cereals, including rice. Gibberellins (GAs) are plant hormones that promote plant growth and developmental processions such as stem elongation and grain size. WRKYs are transcription factors that regulate stress tolerance and plant development including height and grain size. However, the relationship between GA signaling and WRKY genes is still poorly understood. Here, we characterized a small grain and semi-dwarf 3 (sgsd3) mutant in rice cv. Hwayoung (WT). A T-DNA insertion in the 5′-UTR of OsWRKY36 induced overexpression of OsWRKY36 in the sgsd3 mutant, likely leading to the mutant phenotype. This was confirmed by the finding that overexpression of OsWRKY36 caused a similar small grain and semi-dwarf phenotype to the sgsd3 mutant whereas knock down and knock out caused larger grain phenotypes. The sgsd3 mutant was also hyposensitive to GA and accumulated higher mRNA and protein levels of SLR1 (a GA signaling DELLA-like inhibitor) compared with the WT. Further assays showed that OsWRKY36 enhanced SLR1 transcription by directly binding to its promoter. In addition, we found that OsWRKY36 can protect SLR1 from GA-mediated degradation. We thus identified a new GA signaling repressor OsWRKY36 that represses GA signaling through stabilizing the expression of SLR1. Keywords DELLA-like inhibitor · GA signaling · Grain size · Oryza sativa · WRKY transcription factor Jie Lan, Qibing Lin and Chunlei Zhou have contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11103-020-01049-0) contains supplementary material, which is available to authorized users. * Ling Jiang [email protected] * Jianmin Wan [email protected]; [email protected] 1
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China
2
National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
3
Department of Biology and Agricultural Engineering, Quynhon University, Quynhon, Binhdinh 590000, Vietnam
Abbreviations TF Transcription factor cv Cultivar OX Overexpression RNAi RNA interference GFP Green fluorescent protein qRT-PCR Quantitative real-time PCR EMSA Electrophoretic mobility shift assay
Introduction Red
Data Loading...
