Smoking and Physical Activity Significantly Influence Stromal Vascular Fraction Cell Yield and Viability
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ORIGINAL ARTICLE
BASIC SCIENCE/EXPERIMENTAL
Smoking and Physical Activity Significantly Influence Stromal Vascular Fraction Cell Yield and Viability Katarina Andjelkov1
•
Alexandra Conde-Green2 • Afshin Mosahebi3
Received: 20 June 2020 / Accepted: 4 October 2020 Ó Springer Science+Business Media, LLC, part of Springer Nature and International Society of Aesthetic Plastic Surgery 2020
Abstract Background Subcutaneous tissue is an abundant source of adipose-derived regenerative cells. It is readily available and easy to extract by means of liposuction, making it one of the most popular sources for tissue engineering and regenerative medical applications. Methods The stromal vascular fraction (SVF) cell yield and viability of the lipoaspirate obtained from 43 patients undergoing elective liposuction were examined in correlation with their age, gender, body mass index, smoking status, and physical activity. The lipoaspirate was processed with the CelutionÒ 800/CRS system to isolate the SVF and a few drops of the obtained pellet were used for cell counting with NecleoCounterÒ NC-100TM. Results Twenty-eight (65.1%) were men and 15 (34.9%) were women with an average age of 40.7 ± 10.4 years (women) and 38.9 ± 11.8 years (men). Viable SVF cells/ g fat was significantly correlated with smoking level (negative correlation, q= - 0.312, P \ 0.05) and with marginal significance with female gender. Cell viability showed a significant negative correlation with physical activity level (q = - 0.432, P \ 0.01); borderline significance for correlation of this parameter with smoking level should not be neglected. Other parameters did not influence the cell yield nor the viability of the stromal vascular fraction.
& Katarina Andjelkov [email protected] 1
Faculty of Medicine, University of Belgrade, BelPrime Clinic, 16 Brane Crncevica, 11000 Belgrade, Serbia
2
Boca Raton, Florida, USA
3
University College of London, London, UK
Conclusion Many factors may influence SVF cell yield and viability. Our findings indicate that age and smoking significantly influenced SVF cell yield, age positively while smoking negatively. Increased physical activity had a negative correlation with SVF cell viability. Level of Evidence IV This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266. Keywords Stromal vascular fraction Cell yield Cell viability Influence of daily habits Physical activity Smoking
Introduction Regenerative therapies are increasing based on cell-based interventions [1]. Among all available regenerative cells, there has been the highest interest to obtain stromal vascular fraction cells (SVF) from adipose tissue, as they are abundant and easy to harvest. Their clinical benefits, role in fat graft retention, tissue regeneration and tissue engineering have already been established [2]. Nearly 600 cell therapy clinical tr
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