Cell Viability Assays Methods and Protocols
This volume provides an overview of commonly used methods and protocols for cell fitness indicators. Chapters detail biochemical, fluorescence and luminescence-based strategies, computational, and label-free methodologies for assaying cellular viability b
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Daniel F. Gilbert Oliver Friedrich Editors
Cell Viability Assays Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Cell Viability Assays Methods and Protocols
Edited by
Daniel F. Gilbert Friedrich-Alexander University (FAU) Erlangen-Nürnberg, Institute of Medical Biotechnology, Erlangen, Germany
Oliver Friedrich Friedrich-Alexander University (FAU) Erlangen-Nürnberg, Institute of Medical Biotechnology, Erlangen, Germany
Editors Daniel F. Gilbert Friedrich-Alexander University (FAU) Erlangen-Nürnberg Institute of Medical Biotechnology Erlangen, Germany
Oliver Friedrich Friedrich-Alexander University (FAU) Erlangen-Nürnberg Institute of Medical Biotechnology Erlangen, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6959-3 ISBN 978-1-4939-6960-9 (eBook) DOI 10.1007/978-1-4939-6960-9 Library of Congress Control Number: 2017936200 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface In vitro assessment of cellular viability has become a generic approach in addressing a vast range of biological questions in many areas of biomedical research. The spectrum of available cell viability indicators assessing individual physiological, structural, or functional parameters is large and is continuously increasing with the availability and optimization of new or existing technologies. Depending on the number and diversity of employed fitness indic
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