SNHG17 promotes the proliferation and migration of colorectal adenocarcinoma cells by modulating CXCL12-mediated angioge
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PRIMARY RESEARCH
Cancer Cell International Open Access
SNHG17 promotes the proliferation and migration of colorectal adenocarcinoma cells by modulating CXCL12‑mediated angiogenesis Yang Liu1,3* , Qinshan Li3, Dongxin Tang1, Mengxing Li2, Peng Zhao2, Wenxiu Yang2, Liping Shu3, Jishi Wang2, Zhixu He3,4, Yanju Li2* and Feiqing Wang1*
Abstract Background: Colorectal adenocarcinoma (CRA) is one of the leading causes of cancer-related deaths in the world. Long non-coding RNAs (lncRNAs) have been implicated to be effective regulators in the disease course of human cancers, including CRA. Small nucleolar RNA host gene 17 (SNHG17) belongs to lncRNAs, and it has been reported in breast cancer and gastric cancer. However, the function of SNHG17 and its mechanism in CRA progression remain largely unknown. In this study, we attended to shedding some light on the role of SNHG17 in CRA. Methods: RT-qPCR was used to assess SNHG17 expression in CRA cells. CCK-8 assay, colony formation and transwell assay were carried out to detect the regulatory effect of SNHG17 silencing on CRA cell proliferation and migration. The angiogenesis of SNHG7-downregulated CRA cells was analyzed by tube formation assay. Mechanism experiments were conducted to identify the interaction between miR-23a-3p and SNHG17 or C-X-C motif chemokine ligand 12 (CXCL12). Results: SNHG17 possessed with high expression in CRA cells. Knockdown of SNHG17 caused the inhibition on CRA cell proliferation and migration. SNHG17 promoted CRA cell proliferation and migration by sponging miR-23a-3p to upregulate CXCL12. Conclusion: SNHG17 promotes the proliferation and migration of CRA cells by inhibiting miR-23a-3p to modulate CXCL12-mediated angiogenesis. Keywords: SNHG17, miR-23a-3p, CXCL12, Angiogenesis, Colorectal adenocarcinoma
*Correspondence: [email protected]; [email protected]; [email protected] 1 Department of Science and Education, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, No. 71 Bao Shan North Road, Yunyan District, Guiyang 550001, Guizhou, China 2 Department of Hematology, Affiliated Hospital of Guizhou Medical University, No. 28 Guiyi Street, Yunyan District, Guiyang 550004, Guizhou, China Full list of author information is available at the end of the article
Background Colon cancer (CC) can be pathologically divided into colorectal adenocarcinoma (CRA), mucinous adenocarcinoma and undifferentiated cancer, among which CRA accounts for the majority of all CC cases [1–3]. CRA refers to a malignant tumor originally occurring in colon epithelial cells, which possesses with significantly high mortality each year [4]. There is a report reflected that CRA mainly occurs in the group of older people [5]. Some adjuvant therapeutic methods such as chemotherapy and radiotherapy are widely used to decrease
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