Spray-Dried Rosuvastatin Nanoparticles for Promoting Hair Growth
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Research Article Spray-Dried Rosuvastatin Nanoparticles for Promoting Hair Growth Amr Maged,1,5
Azza A. Mahmoud,1,2 Salwa Salah,3 Nehad M. Abd-elmonsif,4 and Mahmoud M. Ghorab3
Received 24 March 2020; accepted 5 July 2020 Abstract.
In this research, we examined the effect of rosuvastatin calcium-loaded nanoparticles on the hair growth–promoting activity on Albino rats. Nanoparticles were prepared using 2:1 weight ratio of drug to methyl-β-cyclodextrin with 10, 20, and 30% stabilizers (phospholipid, polyvinyl pyrrolidone K30, and Compritol 888 ATO) using nanospray dryer. Subsequently, the prepared nanoparticles were evaluated for their process yield, particle size, polydispersity index, zeta potential, and in vitro drug release as well as in vivo studies. The dried nanoparticles showed process yield values up to 84% with particle size values ranging from 218 to 6258 nm, polydispersity index values ranging from 0.32 to 0.99, and zeta potential values ranging from − 6.1 to − 11.9 mV. Combination of methyl-βcyclodextrin with 10% polyvinyl pyrrolidone K30 accomplished nanoparticles with the lowest particle size (218 nm) and polydispersity index (0.32) values. These nanoparticles had suitable process yield value (70.5%) and were able to retard drug release. The hair growth– promoting activity for the selected nanoparticles revealed the highest hair length values in Albino rats after 14 days of the hair growth study compared with non-medicated nanoparticles, nanoparticles’ physical mixture, rosuvastatin solution, and marketed minoxidil preparation groups as well as the control group. The immunohistochemistry images for both selected nanoparticles and marketed minoxidil groups showed a significant increase in the diameter of hair follicle and percent area fraction of cytokeratin 19 in the outer root sheath of hair follicle compared with other tested groups. Rosuvastatin nanoparticles prepared by nanospray drying technique could be a good competitor to minoxidil for hair growth– promoting activity. KEY WORDS: nanoparticles; nanospray dryer; statins; hair growth promotion.
INTRODUCTION The process of hair growth consists mainly of three stages starting with anagen stage, followed by catagen stage and finally telogen stage. Anagen is well known as the growth stage, and it includes the development of hair follicle and proliferation of epithelial stem cells in the niche region of a hair follicle (1,2). Catagen is a regression stage, where the epithelial cells under the niche region start to die (apoptosis) and stop to proliferate. In telogen (resting stage), the hair
1
Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University in Egypt, Cairo, Egypt. 2 Department of Pharmaceutical Technology, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki, Cairo, Egypt. 3 Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Cairo, Egypt. 4 Department of Oral Biology, Faculty of Ora
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