SsPsaH , a H subunit of the photosystem I reaction center of Suaeda salsa , confers the capacity of osmotic adjustment i
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RESEARCH ARTICLE
SsPsaH, a H subunit of the photosystem I reaction center of Suaeda salsa, confers the capacity of osmotic adjustment in tobacco Xiaoyan Hao1,2 · Jianping Li1,2 · Shengqi Gao1,2 · Zumuremu Tuerxun1,2 · Xiaochun Chang1,2 · Wenran Hu1,2 · Guo Chen1,2 · Quansheng Huang1,2 Received: 14 January 2020 / Accepted: 10 July 2020 © The Genetics Society of Korea 2020
Abstract Background Abiotic stress effects agricultural production, so research on improving stress tolerance of crop is important. Suaeda salsa is a halophyte with high salt and drought tolerance and ability to desalinate saline soil and improve soil quality. Objective To discover and utilize of salt and drought tolerance-related genes, we further investigated the mechanisms of salt and drought tolerance. Methods Through screening a salt treated Suaeda salsa cDNA library and further cloning a H subunit of the photosystem I reaction center SsPsaH cDNA, and then the protein domain and phylogenetic analyses of PSI genes was conducted with the NCBI Blast, DNAMAN, and MotifScan programs. The S. salsa seedlings were subjected to various stress treatments and analyze expression of SsPsaH under these treatments by real-time RT-PCR. SsPsaH expression construct was introduced into S. pombe cells by electroporation and transformed into N. tabacum plants by the leaf disc transformation method. Results A member of the H subunit of the Photosystem I reaction center (defined as SsPsaH) was obtained. The expression of SsPsaH was up-regulated by abscisic acid (ABA), salt, and drought stress treatments. Over-expressing SsPsaH in recombinant yeasts enhanced high salinity tolerance and increased tolerance to sorbitol during seed germination and seedling root development in tobacco, respectively. Some stress-related mark genes such as a LEA family gene of NtLEA, a binding protein of a drought response element of NtDREB, the ascorbate peroxidase gene (NtAPX) were also up-regulated in SsPsaH overexpressing transgenic tobacco lines. Conclusions These results show that SsPsaH may contribute to the salt and osmotic stress response of plants. Keywords Photosystem I · The H subunit of the Photosystem I reaction center(PsaH) · Suaeda salsa · Stress tolerance
Introduction Plants are subject to various environmental stresses that adversely influence plant growth and development, such as salt, drought and extreme temperature. These abiotic stresses may lead to reduced plant photosynthetic rates and limiting Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13258-020-00970-4) contains supplementary material, which is available to authorized users. * Quansheng Huang [email protected] 1
Institute of Nuclear and Biological Technology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
Xinjiang Key Laboratory of Crop Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
2
plant growth and development. Adaptation of plant to environment
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