Stable isotopes changes in the adductor muscle of diseased bivalve Ruditapes philippinarum
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ORIGINAL PAPER
Stable isotopes changes in the adductor muscle of diseased bivalve Ruditapes philippinarum Ce´cile Dang Æ X. de Montaudouin Æ N. Savoye Æ N. Caill-Milly Æ P. Martinez Æ P. G. Sauriau
Received: 6 November 2008 / Accepted: 4 December 2008 / Published online: 24 December 2008 Ó Springer-Verlag 2008
Abstract In this article, we show how a disease could bias stable isotope analyzes of trophic networks and propose a strategy in the choice of tissues to be analyzed. In the past few years, a new pathology (brown muscle disease or BMD) affecting the posterior adductor muscle of Ruditapes philippinarum has emerged in Arcachon Bay. BMD induces a necrosis of muscle tissues which become infused by conchiolin and hence calcified. As muscle of mollusks are often used for trophic food webs studies through stable isotopic analyzes, this work investigated the effect of BMD on carbon and nitrogen isotopic ratios of anterior and posterior adductor muscles of clams collected
Communicated by U. Sommer. C. Dang (&) X. de Montaudouin N. Savoye Universite´ de Bordeaux, UMR 5805, Station Marine d’Arcachon, 2 rue du Pr Jolyet, 33120 Arcachon, France e-mail: [email protected] C. Dang X. de Montaudouin N. Savoye CNRS, UMR 5805, Station Marine d’Arcachon, 33120 Arcachon, France N. Caill-Milly IFREMER, Laboratoire Ressources Halieutiques Aquitaine, 64600 Anglet, France P. Martinez Universite´ de Bordeaux, UMR 5805, 33405 Talence, France P. Martinez CNRS, UMR 5805, 33405 Talence, France P. G. Sauriau CNRS, LIENSs, Universite´ de La Rochelle, 17000 La Rochelle, France
in February and August 2007. Infected clams displayed a lower condition index and a posterior adductor muscle d13C enrichment of 1.2% in February and 0.7% in August. d15N of posterior muscles was however not affected by the disease. Anterior muscle of diseased clams remained healthy and displayed the same isotopic signature as both posterior and anterior muscular tissues of healthy clam. Acidification significantly depleted d13C in posterior muscles of infected clams, suggesting calcification, contrary to anterior muscles of infected clam and to both muscles of healthy clams, where no effect was observed. An X-ray diffractometry analysis confirmed the presence of CaCO3 (aragonite). Trophic food web studies relying on stable isotope ratios should utilize only healthy animals or anterior adductor muscles when expertise in mollusk pathology is lacking.
Introduction The Manila clam Ruditapes philippinarum (Adams and Reeve 1850) originates from Indo–Pacific waters and nowadays contributes to more than half of global yields of clams. Since 1930, Manila clam has been introduced with Pacific oyster (Crassostrea gigas) seed into different parts of the world, e.g., from the United States to Canada and to the Hawaiian islands (Flassch and Leborgne 1992). In Europe, R. philippinarum was primarily introduced into France for culture purposes in 1972 and later to England, Spain and Italy (Flassch and Leborgne 1992). Within a few years, the species established nat
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