The study of the adductor muscle-shell interface structure in three Mollusc species
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The study of the adductor muscle-shell interface structure in three Mollusc species ZHU Yaoyao1, 2†, SUN Chengjun1, 3†, SONG Yingfei2, JIANG Fenghua1, YIN Xiaofei1, TANG Min4, 5, DING Haibing6, 7* 1 Marine Ecology Center, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China 2 College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China 3 Laboratory of Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology,
Qingdao 266071, China 4 Key Laboratory of Advanced Materials of Tropical Island Resources of Ministry of Education, Hainan University,
Haikou 570228, China 5 College of Material and Chemical Engineering, Hainan University, Haikou 570228, China 6 Key Laboratory of Marine Chemistry Theory and Technology of Ministry of Education, Ocean University of China,
Qingdao 266100, China 7 Qingdao Collaborative Innovation Center of Marine Science and Technology, Ocean University of China, Qingdao
266100, China Received 11 May 2015; accepted 14 September 2015 ©The Chinese Society of Oceanography and Springer-Verlag Berlin Heidelberg 2016
Abstract
The adductor muscle scar (AMS) is the fixation point of adductor muscle to the shell. It is an important organicinorganic interface and stress distribution area. Despite recent advances, our understanding of the structure and composition of the AMS remain limited. Here, we report study on the AMS of three bivalves: Mytilus coruscus, Chlamys farreri and Ruditapes philippinarum. Results showed that there were significant differences among their AMS structures. Both M. coruscus and C. farreri were found to have a columnar layer above the nacreous platelet shell structure at the AMS and this layer was more organized in M. coruscus. There was no distinguishable twolayer structure in R. philippinarum. Atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FT-IR) results showed that the AMS was much smoother than the nacreous inner shell in all the three species and the AMS had minor different compositions from the nacreous shell layer. SDS-PAGE (sodium dodecyl-sulfate polyacrylamide gel electophoresis) study of the proteins isolated from the interface indicated that there was a 70 kDa protein which seemed to be specifically located to the highly organized columnar AMS structure in Mytilus coruscus. Further analysis of this protein showed it contained high level of Asx (Asp+Asn), Glx (Glu+Gln) and Gly. The special structure and composition of the AMS might play important roles in the stability, adhesion and function at this stress distribution site. Key words: mollusc shell, adductor muscle-shell interface, shell protein, shell microstructure Citation: Zhu Yaoyao, Sun Chengjun, Song Yingfei, Jiang Fenghua, Yin Xiaofei, Tang Min, Ding Haibing. 2016. The study of the adductor muscle-shell interface structure in three Mollusc species. Acta Oceanologica Sinica, 35(8): 57–64, doi: 10.1007/s13131-016-0878-x
1 Introduction Mollusc shells have been regarded as artware
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