Statistical Optimization of Parameters for Enhanced Bioactive Metabolites Produced by Streptomyces hygroscopicus AVS7
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RESEARCH ARTICLE-BIOLOGICAL SCIENCES
Statistical Optimization of Parameters for Enhanced Bioactive Metabolites Produced by Streptomyces hygroscopicus AVS7 Manon Mani Vellingiri1,3 · Parimala Gnana Soundari Arockiam Jeyasundar2,3 · Brindha Priyadarisini Venkatesan3 · Wen-chao Liu4 · Balamuralikrishnan Balasubramanian5 Received: 30 July 2020 / Accepted: 2 November 2020 © King Fahd University of Petroleum & Minerals 2020
Abstract The modern technology focuses on developing a novel statistical approach for highest yield of bioactive microbial metabolites which contain therapeutic properties, eventually leading to a promising strategy for treating various diseases and disorders. In this research, we aimed to develop the optimization process for maximum yield of crude pigmented secondary metabolites (CPSM) from Streptomyces hygroscopicus AVS7 through statistical approaches and determine its antimicrobial activity. The isolate produced maximum of 2.92 g/L on 9th day in starch casein nitrate (SCN) medium, and employing the SCN medium as seed medium, the research has initiated one factor at a time (OFT) for screening the essential physical, physiological and biological parameters. The OFT-screened factors were further determined for their importance through Plackett–Burman (PB) design, and the PB results revealed the significant factors (sesame oil cake, KNO3 , glycerol, glucose, casein and urea). The experimentation was carried to Box-Behnken (BB) design–RSM using significant factors for the evaluation of interaction between the factors influencing the highest yield of CPSM, and the model was validated. The maximum CPSM extract was yielded by culturing the S. hygroscopicus AVS7 in the optimized medium, and there was twofold increase in the yield of CPSM extract when compared to seed medium. This statistical approach is a low-cost and economical method to develop maximum CPSM for medicinal and industrial purposes. The produced CPSM was found to contain the antimicrobial properties. Further, the research leads to the in vitro and in vivo assessment of purified bioactive secondary metabolite. Keywords Antimicrobial · Box–Behnken design · Metabolites · Optimization · Plackett–Burman design · Streptomyces sp.
1 Introduction Manon Mani Vellingiri, Parimala Gnana Soundari Arockiam Jeyasundar, Wen-chao Liu and Balamuralikrishnan Balasubramanian have equally contributed as first author.
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Brindha Priyadarisini Venkatesan [email protected] Balamuralikrishnan Balasubramanian [email protected]
1
Department of Biotechnology, Rathnavel Subramaniam College of Arts and Science, Coimbatore, Tamil Nadu 641402, India
2
Department of Environmental Science & Engineering, College of Natural Resources & Environment, Northwest A & F University, Shaanxi 712100, China
3
Department of Microbial Biotechnology, Bharathiar University, Coimbatore, Tamil Nadu 641046, India
4
Department of Animal Science, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, People’s Republic of China
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