Stimulating living cells with air DBD plasma
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Stimulating living cells with air DBD plasma P. Favia 1,2,3, D. Pignatelli1 , G. Dilecce2, B. R. Pistillo1 , M. Nardulli1, R. Gristina2 1
Department of Chemistry, University of Bari “A. Moro”, Italy
2
CNR-Institute of Inorganic Methodologies and Plasmas, IMIP-CNR, Bari, Italy
3
Plasma Solution Srl, Spin Off dell’Università di Bari “A. Moro”, Italy
ABSTRACT Cells positioned at the bottom of a Petri dish were exposed, in a home-made plasma source, to pulsed Dielectric Barrier Discharges operated in air in order to investigate the effect of the plasma species on their viability and growth. Processes with different number of pulse, respectively 1,3,9 and 27 pulse, were performed to study the influence on viability and cell growth of two different cell lines, Saos 2 and NHDF. Atmospheric air discharges applied on the two selected cell lines have shown an effect strongly dependent on cell type. At certain doses we have measured increased activity of the NHDF fibroblasts cell line. On the other side, an inhibition of cell adhesion and growth on the Saos 2 osteoblastoma cell line, directly dependent on the plasma doses, was clear. This study shows that by properly tuning the dose of exposure of cells to air plasma it is possible to induce both positive and negative effects on cell growth, that would be useful in several branches of Medicine. INTRODUCTION Cold plasmas at low/atmospheric pressure are widely utilized since almost 50 years to modify the surface of materials intended to be used for biomedical applications, with the aim of driving the interactions of proteins, cells, and biological tissues with materials in lab wares, prostheses, biomedical devices and the like. In the last 20 years plasmas have also been used for sterilization and decontamination of surfaces of biomedical interest. More recently, in the new field of Plasma Medicine [1,2,3], direct exposure of living tissues to atmospheric pressure air plasmas have started to be utilized for therapeutic uses, e.g. for sterilization and decontamination of wounds [4,5], for wound healing [6], for teeth bleaching and disinfection of teeth root canals[7], for blood coagulation [8], for cancer therapy [9], and for other uses [10]. The mechanisms by which low-temperature atmospheric pressure plasma affect viruses, bacteria and eukaryotic cells are based on the synergy of several biologically active plasma components like plasma-generated UV radiation and reactive species (RS) including free radicals molecules such as peroxides and ozone[11]. Such interactions can involve lethal or positive effects on living cell [12]. In order to understand the mechanisms underlying the different interaction between plasma and eukaryotic cells, in vitro experiments with cell lines represent a very powerful tool. In this work the selective effects of different doses of DBD (Dieletric Barrier Discharge) air plasma on different cell lines, an immortal one, SAOS-2 osteoblastoma, and a primary one, NHDF fibroblasts, were investigated.
EXPERIMENTAL DETAILS The homemade plasma source
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