Structural and functional genomics analysis of methyltransferase genes and networks associate to understand antibiotic r
Multi-drug resistant Pseudomonas aeruginosa has multiple issues, all associated with the diverse genetic and epigenetics traits present in this pathogen, ranging from multi-drug resistant genes to the molecular machinery for the biosynthesis of biofilms d
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University of Antioquia, Biology Institute, FCEN, Genetics and Biochemistry of Microorganisms group (GEBIOMIC), address: calle 67 No. 53 – 108, block 7, office: 318, Medellín, Colombia
Abstract—Multi-drug resistant Pseudomonas aeruginosa has multiple issues, all associated with the diverse genetic and epigenetics traits present in this pathogen, ranging from multidrug resistant genes to the molecular machinery for the biosynthesis of biofilms developed by quorum sensing. In this work was established bioinformatics strategy to study resistance inside the pangenome and compared with Colombian resistant strains. Using the information hosted in PATRIC database of P. aeruginosa from 200 genomes, was very important to understand the genomics structure and the relationship between antibiotic resistance associated to rsmH, rsmG and rsmI methyltransferases genes and proteins. This bioinformatics analysis and the sequencing of rsmG, rsmH and rsmI in P. aeruginosa from Colombia, was useful, establishing a correlation inside the pangenome from this bacteria. There is an association between genomic structure and function to draw together antibiotic resistance with those genes in the pangenome. Keywords— Bioinformatics, pangenome, methyltransferase, antibiotic resistance, multi-drug resistant, structural and functional genomics. I. INTRODUCTION
Major issues in the development of a successful Pseudomonas aeruginosa vaccine arise from the probable genotypic variation at the strain level, making P. aeruginosa a presumably antigenically variable organism. Results supporting this assumption have been reported, yielding genetic information from the P. aeruginosa genome. For example, genetic variability explored in multiple P. aeruginosa isolates from different regions of the world indicated that pcrV, a member of the type III secretion system, exhibits limited genetic variation in terms of non-synonymous substitutions [1]. Previously, it was constructed a pangenome of P.aeruginosa [2]. The P. aeruginosa pangenome was estimated to contain more than 16,000 non-redundant genes, and approximately 15 % of these constituted the core genome. Functional analyses of the accessory genome indicated a wide presence of genetic elements directly associated with pathogenicity. Now we want to emphasize in antibiotic resistance.
In recent years have become important scrutinize on the molecular function of the ribosome and its importance in the processes of antibiotic resistance and post-translational modifications that occur in the rRNAs and tRNAs were involved in their function [3]. Of particular interest is the study of the methylations present in the bacterial 16S rRNA and 23S, because mechanisms are established in several human pathogens such as P. aeruginosa to acquire resistance to antibiotics. One interesting group of proteins acting as RNAmodifying enzymes is composed of AdoMet- (or S-adenosylL-methionine) dependent RNA methyltransferases (MTases). Globally, enzymes that methylate RNA comprise two major classes of MTases according to their st
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