Studies on Biocementation of Mortar and Identification of Causative Bacteria
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RESEARCH ARTICLE-CIVIL ENGINEERING
Studies on Biocementation of Mortar and Identification of Causative Bacteria Anuja U. Charpe1
· Snigdha P. Bhutange2 · M. V. Latkar2 · T. Chakrabarti3
Received: 7 May 2020 / Accepted: 15 October 2020 © King Fahd University of Petroleum & Minerals 2020
Abstract A major challenge for construction industry is the durability of structures. A lot of work is being done to strengthen structures. Microbially induced calcium carbonate precipitation (MICCP) or biocementation is an innovative technology, which uses ureolytic bacteria to precipitate CaCO3 to strengthen cementitious materials. In this research, the effect of a bio-solution incorporated with soil as microbial source on the mechanical properties of mortar was studied. Cost reduction in the process was done by using natural ingredients. A considerable increment in compressive strength and a decrement in water absorption of the bio-mortar specimens were observed. Two ureolytic bacteria were identified, viz. Bacillus cereus and Enterobacter cloacae. The effect of these identified ureolytic pure cultures on mortar was also studied. Keywords Bio-mortar · Compressive strength · Bacillus cereus · Enterobacter cloacae
1 Introduction The demand of cementitious materials is ever increasing due to constant developments in the civil engineering field and growing industrial activities. A structure is desired to be strong and long lasting as it is expected to withstand many environmental challenges. The durability of a structure is a matter of rising concern in this modern era with the increase in building construction activity. A variety of epoxy and synthetic filler agents are available for aug-
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Anuja U. Charpe [email protected] Snigdha P. Bhutange [email protected] M. V. Latkar [email protected] T. Chakrabarti [email protected]
1
Civil Engineering Department, Velagapudi Ramakrishna Siddhartha Engineering College, Vijayawada, Andhra Pradesh 520007, India
2
Civil Engineering Department, Visvesvaraya National Institute of Technology (VNIT), Nagpur, Maharashtra 440010, India
3
Maharashtra Pollution Control Board, Civil Engineering Department, Visvesvaraya National Institute of Technology (VNIT), Nagpur, Maharashtra 440010, India
menting the service life of a structure. There is a need of cost-effective and ecofriendly technology to replace the conventional technology [1]. The use of microorganisms in the field of construction has become an important research area. Some types of bacteria produce CaCO3 through their natural metabolism, and the CaCO3 gets precipitated. This phenomenon is known as microbially induced calcium carbonate precipitation (MICCP) or biocementation [2, 3]. The urease enzyme from the bacteria carries out the hydrolysis of urea to form carbonate ions. If calcium-containing compound is present, CaCO3 is produced which gets precipitated. Thus, the bacteria precipitate calcium carbonate that can improve the properties of cementitious materials and can act as an effective binder and a high-qual
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