Species identification and mutation breeding of silicon-activating bacteria isolated from electrolytic manganese residue
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RESEARCH ARTICLE
Species identification and mutation breeding of silicon-activating bacteria isolated from electrolytic manganese residue Ying Lv 1
&
Jia Li 1,2 & Zhenxing Chen 1 & Hengpeng Ye 1 & Dongyun Du 1 & Li Shao 1 & Mengyu Ma 1
Received: 8 June 2020 / Accepted: 13 August 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A strain of silicon-activating bacteria was isolated from electrolytic manganese residue (EMR); identified as a species of Ochrobactrum by integrated microscopic morphological characteristics, biochemical index determination, and clone analysis (i.e., results of 16S rRNA sequence); and temporarily named as Ochrobactrum sp. T-07 (T-07). The optimal growth conditions of the strain T-07 were obtained as follows: temperature of 30 °C, initial pH of 7.0, shaking speed of 180 rev. min−1, and loading volume of 100 mL. In order to enhance its activation activity of silicon, T-07 went through the ultraviolet (UV) mutagenesis and nitrosoguanidine (NTG) mutagenesis breeding, and the mutant strain T-07-B with higher activity was obtained. Under the optimal fermentation condition (leaching time of 20 days, temperature of 30 °C, initial pH of 7, pulp concentration of 5%, shaking speed of 180 rev. min−1, and particle diameter of EMR ≤ 180 μm), the available silicon content in the supernatant reached 98.8 mg L−1, which was 2.4 times of the original strain T-07. Therefore, T-07 can be used as a good backup in developing biological silicon fertilizer for plants. Keywords Electrolytic manganese residue . Silicon-activating bacteria . Ochrobactrum sp. . Species identification . Mutagenesis breeding
Introduction Highlights • A strain of silicon-activating bacteria named T-07 was isolated from EMR. • The activation activity of silicon was enhanced after mutagenesis breeding. • The optimum fermentation condition of T-07-B was investigated. • T-07 can be used as a good backup of biological silicon fertilizer development. Responsible editor: Diane Purchase Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11356-020-10526-4) contains supplementary material, which is available to authorized users. * Jia Li [email protected] 1
College of Resource and Environmental Science, South-Central University for Nationalities, Wuhan 430074, People’s Republic of China
2
School of Forestry & Environmental Studies, Yale University, New Haven, CT 06511, USA
Electrolytic manganese residue (EMR) is a kind of solid waste produced in the process of electrolytic manganese production. The enormous accumulation of EMR easily causes serious environmental pollution, thus restricting the benign development of electrolytic manganese industry (Lan et al. 2019; Tang et al. 2019; Wang et al. 2019). At the same time, there are many beneficial elements in EMR. The key to the sustainable development of the electrolytic manganese industry is to recycle and reuse these elements with reasonable means to make EMR have economic benefits and to reduce EMR treatment (Shu e
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