Studies on qualitative and quantitative characters of mutagenised chili populations induced through MMS and EMS
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RESEARCH ARTICLES
Studies on qualitative and quantitative characters of mutagenised chili populations induced through MMS and EMS Nazarul Hasan1 · Sana Choudhry1 · Rafiul Amin Laskar2 Received: 27 June 2020 / Accepted: 19 September 2020 / Published online: 2 November 2020 © Society for Plant Research 2020
Abstract Capsicum annum L. is the one of the economically important spice crop and has been in use from the ancient time for both food and medicinal purposes. The present investigation has been carried out to study the responses of C. annum, to the chemical mutagens MMS and EMS (0.1%, 0.25%, 0.50%, 0.75% and 1.0%). The qualitative and quantitative traits were observed from the seedling stage to maturity of chili plant, which includes plant height, leaf size and leaf shape, branch per plant, fruit size, number of pods per plants, weight of 1000 seeds in gram, yield per plant and root length. Statistical analysis of the traits showed the induction of significant variations at different concentrations of the mutagen treatments. Quantitative traits including plant height and yield per plant showed useful variations for further selection and advancement in the subsequent generations. The observed morphological variations provided valuable genetic resources which will be helpful in improving the agronomic characters through chili breeding program. Keywords Capsicum annum L. · Methylmethane sulphonate (MMS) · Ethylmethane sulphonate (EMS) · Induced mutagenesis · Quantitative traits · Qualitative traits
Introduction Capsicum annum L. (2n = 24) is a member of solanaceae family. C annum and the other members of the genus Capsicum constitute an important spice around the world. Alkaloid capsaicin is obtained from the chili plant and pungency of chili has great importance in tear gas and help in preparation of culinary (Sanatombi and Sharma 2008; MansourGueddes et al. 2010; Smith and Brooks 2014). Chiliis, the source of vitamins (A, B, C and E) and different minerals elements may contain elements like molybdenum, potassium, thiamine, foliate, manganese and copper. The genus Capsicum, comprises the 28–30 species of chili and some economically important species, domesticated and grown throughout the world, includes C. annum, C. baccatum, C. fruitscence, C. pubescence and C. chinenses (Bosland and Votava 2000; Sudré et al. 2010; Ibiza et al. * Rafiul Amin Laskar [email protected] 1
Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh 202002, India
Department of Botany, Bahona College, Jorhat, Assam 785101, India
2
2012; Eshbaugh 2012). Among the five species of Capsicum genus, C. annum is cultivated throughout the world, (Carrizo et al. 2016; Aguilar-Meléndez et al. 2009) followed by C. frutescens (Ince et al. 2010; Wang et al. 2006; Bosland et al. 2012; Schweiggert et al. 2006). Capsicum annum has been classified into four cultivar-groups namely: Sweet pepper, chili, bird pepper and aromatic pepper (Grubben et al. 2004). Although, chili grows all over the world but India is the largest producer o
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