Study of Protein Adsorption onto a Polymer Film by in-situ UV Attenuated Total Reflectance Spectroscopy
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1138-FF03-15
Study of Protein Adsorption onto a Polymer Film by in-situ UV Attenuated Total Reflectance Spectroscopy 1
Maria Antoaneta Bratescu1, Syohei Fujita2, Nagahiro Saito3 and Osamu Takai1 EcoTopia Science Institute, Nagoya University, 2Department of Materials, Physics and Energy Engineering, Nagoya University, 3Department of Molecular Design and Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan
ABSTRACT Our work presents results on human plasma protein adsorption onto a polyacrylic acid (PAA) film prepared via surface wave plasma (SWP) induced graft polymerization. The PAA film prepared in this manner is characterized by a carboxyl functional group and a constant contact angle in water of 35°. The adsorption kinetics of human plasma fibrinogen (HPF) and human serum albumin (HSA) proteins were measured by in-situ UV-ATR spectroscopy. The free energy of adsorption on PAA treated as well as untreated surfaces was –28 kJ M-1 and –22 kJ M1 for HPF and HSA, respectively, regardless of surface chemistry. We determined that 14 µM and 6 µM HPF concentrations are enough to cover half of the maximum possible of surface coverage on silica and PAA film, respectively. HSA protein concentrations of 154 µM and 118 µM are enough to cover half of the maximum accessible surface of silica and PAA film, respectively. For surface treatment of implants with PAA polymer and protein, the necessary protein concentration for effective surface coverage should be known. INTRODUCTION Recently graft chains polymer films and densely packed or charged polymer brushes have been used to control the adsorption process of a protein on a surface. Surface grafting polymerization on various substrates is mostly used to improve the surface properties such as wettability, the antistatic property and adsorption of biomolecules without deterioration of the bulk characteristics. In materials used for implants, cell adhesion and growth can be promoted by immobilization of proteins on polymer films deposited on these materials [1, 2]. The evanescent wave produced in total internal reflection is widely used to study surface and interfacial processes by attenuated total reflectance (ATR) spectroscopy, surface plasmon resonance or total internal reflectance methods [3]. Human plasma protein adsorption behavior on various surfaces still receives considerable interest because of its importance in biological, medical, and industrial applications. Generally, the research is focused on the importance of the surface hydrophobicity given by different functional groups [4], and on the influence of interface material, like glass [5], noble metals [6], titanium [7], or polymers [1]. Our work presents results about the human plasma protein adsorption onto a poly acrylic acid (PAA) film prepared by a new graft polymerization method after surface pre-treatment with a surface wave plasma (SWP) [8]. The adsorption kinetics of human plasma fibrinogen (HPF) and human serum albumin (HSA) proteins were measured by in-situ UV-ATR spectroscopy metho
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