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Quantification of Protein Adsorption on Polyglycerol-based Polymer Network Films Duygu Ekinci1, Adam L. Sisson1, and Andreas Lendlein1 1 Institute of Biomaterial Science and Berlin Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Kantstr. 55, 14513 Teltow, Germany. ABSTRACT Neutral, hydrophilic, polymer-based architectures are widely investigated for a wide range of biomedical applications from drug-conjugates to delivery systems and scaffolds for regenerative therapies. In most cases, it is crucial that biomaterials provide a blank, inert background in order to hinder unspecific cell-material interactions so that protein mediated biological events leading to foreign body reactions are prevented. Hydrophilic polyglycerolbased polymer network films are a recently developed class of amorphous macroscopic materials, which offer great versatility in design and control of resultant properties. In this study, protein adsorption on polyglycerol-based polymer network films is investigated by using Micro BCA protein assay for three types of proteins having critical roles in the human body, and various copolymer networks with differing sidechains and crosslink densities. INTRODUCTION In the course of biomaterial development, functionality is one of the main concerns in order to conceptualize how the material transduces its structural makeup to direct or control the response of the organisms in vivo [1]. It is critical to recognize that the bulk and surface properties of biomaterials used for medical implants have been shown to influence the dynamic interactions that take place at the tissue-implant interface [1]. In construction of biocompatible artificial implants, one of the main strategies to accommodate cell-material interactions is creating an inert surface not allowing the adsorption of proteins and adhesion of cells. It is generally acknowledged that surfaces that resist protein adsorption will also resist cell adhesion [1]. And above all, resistance of such nonfouling surfaces to protein adsorption would prevent a cascade of protein mediated biological events such as activation of the immune system, blood coagulation, thrombosis, extracellular matrix deposition and other interactions between the material and the surrounding environment. It is generally accepted that hydrophilic surfaces possessing high wettability and overall neutral charge generally resist protein adsorption and thus protein mediated biological events. Hydrogels among the vast number of synthetic biomaterials stand as the best candidate to nonfouling surface materials. Poly(ethylene glycol) is one the polymers studied extensively for its antifouling properties, especially when coated on planar model surfaces as self assembled monolayers (SAMs) [2]. In addition, bulk structures based on poly(ethylene glycol) showed antifouling properties [3]. Polymers based on poly(2-hydroxyethyl methacrylate) [4], and poly(vinyl alcohol) [5] are also among some of the bulk structures studied for their protein repellent properties. Notably,