Study on the immobilization of hemoglobin by nano mesoporous MCFs and its optical properties
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Study on the immobilization of hemoglobin by nano mesoporous MCFs and its optical properties Qing‑Shuang Wang1,2 · Chao‑Qun Song1 · Qing‑Zhou Zhai2 Received: 23 February 2019 / Revised: 29 May 2019 / Accepted: 27 June 2019 © Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract Nano mesoporous molecular sieve MCFs (mesocellular foams) was synthesized successfully through hydrothermal method. Then bovine hemoglobin was successfully immobilized into the mesoporous molecular sieve MCFs, the immobilized enzyme was 50.27 mg g−1. The desirable adsorbate/adsorbent, Hb/MCFs, (mass ratio, m/m) for 3/50 was achieved by adjusting adsorption conditions. The obtained materials were characterized using various techniques such as the powder XRD, Fourier transform infrared spectroscopy, UV–Vis solid diffuse reflectance spectroscopy (SDRS), photoluminescence spectrum and N2 adsorption desorption study at 77 K. The results showed that hemoglobin had been immobilized successfully inside the channels of mesoporous MCFs. Scanning electron microscopy was used to confirm the average particle diameters of MCFsHb and (CH3-MCFs)-Hb. And the results showed that the average particle diameters of MCFs-Hb and (CH3-MCFs)-Hb were 2212 ± 50 nm and 2219 ± 50 nm, respectively. The adsorption process of MCFs/(CH3-MCFs) adsorbing hemoglobin enzyme was in accordance with pseudo-second-order kinetic equation. The process of MCFs/(CH3-MCFs) adsorbing hemoglobin enzyme belonged to an exothermic reaction which was a spontaneous reaction. Freundlich model described the adsorption of hemoglobin by MCFs/CH3-MCFs well. The desorption process of MCFs-Hb/(CH3-MCFs)-Hb composite was studied in the sodium hydroxide solution (0.1 mol L−1), and equilibrium was reached at 5 h. The desorption rates were 72.58% and 69.67%, respectively. The luminescence spectra showed that the obtained composites had the light-emitting properties. Keywords Hemoglobin · Immobilization · Mesocellular foams MCFs · Nano mesoporous molecular sieve · Luminescence
1 Introduction Mesoporous silicate has been widely explored as a carrier of protein and enzyme for biological catalytic application, owing to the ordered pore structure and its size which is comparable to many biological molecules. Because of their original discovery, the novel synthetic methods could control the pore size, grain size, structure, chemical composition Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10450-019-00139-w) contains supplementary material, which is available to authorized users. * Qing‑Zhou Zhai [email protected]; [email protected] 1
School of Life Science and Technology, South Campus, Changchun University of Science and Technology, Changchun 130022, Jilin, China
Research Center for Nanotechnology, South Campus, Changchun University of Science and Technology, Changchun 130022, Jilin, China
2
and stability precisely, so that many kinds of biological macromolecules can be adsorbed into their structures, such as heme p
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