Preliminary study on effect of nano-hydroxyapatite and mesoporous bioactive glass on DNA
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Sabyasachi Chatterjee Biophysical Chemistry Laboratory, Organic and Medicinal Chemistry Division, CSIR-Indian Institute of Chemical Biology, Kolkata 700032, India
Biswanath Kundua) Bioceramics and Coating Division, CSIR-Central Glass and Ceramic Research Institute, Kolkata 700032, India
Gopinatha Suresh Kumar Biophysical Chemistry Laboratory, Organic and Medicinal Chemistry Division, CSIR-Indian Institute of Chemical Biology, Kolkata 700032, India (Received 7 January 2018; accepted 11 April 2018)
In this study, nano-hydroxyapatite (n-HAp) of average crystallite size ;8.15 6 4 nm of hexagonal geometry with size ranging between 14 and 50 nm was synthesized in laboratory at room temperature by using suitable sources of calcium and phosphate ions and using triethanolamine. Mesoporous bioactive glass (MBG) was synthesized by using cationic surfactant cetyl trimethyl ammonium bromide of the SiO2–CaO–P2O5 glass system. After calcination at 650 °C, MBG powders were having a zeta potential of 16.5 mV (pH ;9.1), median particle size ;75 nm, and specific surface area 473.2 m2/g. An aqueous suspension of DNA was used to disperse both n-HAp and MBG and further subjected for analysis including absorbance, circular dichroism spectroscopy, UV-melting, and isothermal titration calorimetry. Absorbance spectroscopy indicated that an equilibrium binding was obtained between both materials and DNA in solution phase. Due to the addition of the nanomaterial, molar ellipticity of DNA was changed revealing that the materials were interacted with DNA. From UV melting characterization, there is a shifting of the melting temperature of DNA in the presence of MBG and n-HAp, respectively, suggesting that the nanoparticles stabilized DNA helix to a considerable extent.
I. INTRODUCTION
Hierarchically, human bone is an excellent example of macro- to micro- to molecular structure, which starts from spongy bone from surface down to collagen fibrils on a length scale, which is again composed of columnar collagen molecule and inorganic component mainly hydroxyapatite (HAp) nanocrystals.1 This structure of bone provides unique mechanical properties to support the body weight, producing different blood cells, and also for storing of different ions.2 Many authors have tried to explain the correlation between this hierarchic structure up to molecular level and mechanical properties observed in different parts of the body through different methods including finite element modeling,3 multiscale modeling techniques,4 etc. Bhowmik et al. had shown the mechanical effect of collagen molecules with mineral and also a)
Address all correspondence to this author. e-mail: [email protected] DOI: 10.1557/jmr.2018.114
effect on the function of the protein biomolecules at the region of inorganic–organic interfaces.1 At the nanoscale level, bone consists of HAp mineralized at specific positions on collagen molecules. This mineralized nanohydroxyapatite (n-HAp) in these regions is occurred as hexagonal geometry with the c-axis parallel to the collagen fibrils5
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