Substrate and film structure impacts on adhesion properties between lignocellulosic polymers
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Substrate and film structure impacts on adhesion properties between lignocellulosic polymers Elias Estephan1,2,3, Véronique Aguié-Béghin2, 3*, Loïc Muraille1, 2, 3 and Michael Molinari1* 1
Laboratoire de Microscopie et d’Etude de Nanostructure, Université de Reims ChampagneArdenne, F-51100 Reims, France. 2 INRA, UMR 614 Fractionnement des AgroRessources et Environnement, F-51100 Reims, France. 3 UMR 614, Fractionnement des AgroRessources et Environnement, Université Reims Champagne-Ardennes, F-51100 Reims, France. * Corresponding authors : E-mail address : [email protected] and [email protected]
ABSTRACT Using AFM, ellipsometric and profilometric measurements, we have investigated the topography and the thickness of the cellulose nanocristal (NCC) films prepared by two different methods: the first one is obtained by evaporation of concentrated suspension of NCC in petridish to produce a self-supported film; the second one is produced by dipping thin NCC layer on silicon substrate by Langmuir-Blodgett (LB) technique. Glucomanan (GM) functionalized AFM tip was brought into contact with these two kinds of NCC films in order to measure the adhesion between GM and NCC. The impact of the substrate and the thickness of NCC films on the measured adhesion forces were also studied: the mean measured adhesion force between the two polysaccharides was 17 nN regardless of the way the films were prepared. Adhesion properties can help us understand biochemical processes in the plant cell wall. INTRODUCTION AFM has numerous features that render it a versatile tool with several advantages over other microscopy techniques, notably for studying biopolymers properties. Despite of the recent years important advances in using AFM for imaging of biomolecules, there is a continuous demand for new microscopic and spectroscopic AFM studies able to provide complementary information on their mechanical properties. Detection up to single-molecule forces is feasible with AFM force-mode; moreover, it allows measuring various biophysical properties of materials, such as elasticity, adhesion, hardness, friction, etc. Also, AFM has been used for the identification of pairs interactions like antigen-antibody [1-2], biotin-streptavidin [3-4]. Polysaccharides are found extensively throughout nature as major components of plant cell walls. Physical and mechanical properties of polysaccharide components of plant cell walls can provide new insights into how cell walls are built, and how they can be used in new high quality fibre-based agromaterials. The plant cell wall has a high degree of structural complexity:
it is mainly constituted of cellulose (linear chain of D-Glucose) which is the most abundant material on earth and serves to maintain the structure of the plant. Glucomannan is, unlike cellulose, an heterogeneous polysaccharide composed of various sugar units (D-mannose and Dglucose). It is tightly bound to cellulose and may be good candidate to be a cross-linker between amorphous matrix polymers and cellulose microfib
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