Suitcase Lab: new, portable, and deployable equipment for rapid detection of specific harmful algae in Chilean coastal w
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RESEARCH ARTICLE
Suitcase Lab: new, portable, and deployable equipment for rapid detection of specific harmful algae in Chilean coastal waters So Fujiyoshi 1,2,3 & Kyoko Yarimizu 1 & Yohei Miyashita 4 & Joaquín Rilling 3 & Jacquelinne J. Acuña 2,3 & Shoko Ueki 4 & Gonzalo Gajardo 5 & Oscar Espinoza-González 6 & Leonardo Guzmán 7 & Milko A. Jorquera 2,3 & Satoshi Nagai 8 & Fumito Maruyama 1,2,3 Received: 25 February 2020 / Accepted: 5 November 2020 # The Author(s) 2020
Abstract Phytoplankton blooms, including harmful algal blooms (HABs), have serious impacts on ecosystems, public health, and productivity activities. Rapid detection and monitoring of marine microalgae are important in predicting and managing HABs. We developed a toolkit, the Suitcase Lab, to detect harmful algae species in the field. We demonstrated the Suitcase Lab’s capabilities for sampling, filtration, DNA extraction, and loop-mediated isothermal amplification (LAMP) detection in cultured Alexandrium catenella cells as well as Chilean coastal waters from four sites: Repollal, Isla García, Puerto Montt, and Metri. A LAMP assay using the Suitcase Lab in the field confirmed microscopic observations of A. catenella in samples from Repollal and Isla García. The Suitcase Lab allowed the rapid detection of A. catenella, within 2 h from the time of sampling, even at a single cell per milliliter concentrations, demonstrating its usefulness for quick and qualitative on-site diagnosis of target toxic algae species. This method is applicable not only to detecting harmful algae but also to other field studies that seek a rapid molecular diagnostic test. Keywords Deployable toolkit . Loop-mediated isothermal amplification . On-site molecular detection . Harmful algal bloom . Plankton monitoring
Introduction Phytoplankton blooms are frequent phenomena observed in coastal regions of every continent in the world. Some of the
blooming species have been found to produce endogenous toxins and such harmful algae blooms (HABs) can, directly and indirectly, cause acute effects on marine and freshwater ecosystems leading to major impacts on public health and
Responsible Editor: Philippe Garrigues * So Fujiyoshi [email protected]
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Institute of Plant Science and Resources, Okayama University, 2-20-1 Chuo, Kurashiki, Okayama 710-0046, Japan
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Laboratorio de Genética, Acuicultura & Biodiversidad. Departamento de Ciencias Biológicas y Biodiversidad, Universidad de Los Lagos, Osorno, Chile
Office of Industry-Academia-Government and Community Collaboration, Hiroshima University, 1-3-2 Kagamiyama, Higashi-Hiroshima City, Hiroshima 739-8511, Japan
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Center for holobiome and built Environment (CHOBE), Hiroshima University, 1-3-2 Kagamiyama, Higashi-Hiroshima City, Hiroshima 739-8511, Japan
Centro de Estudios de Algas Nocivas (CREAN), Instituto de Fomento Pesquero (IFOP), Padre Harter 547, 5480000 Puerto Montt, Chile
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Instituto de Fomento Pesquero, IFOP, Balmaceda 252, 5480000 Puerto Montt, Chile
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Japan Fisheries Research and Education Agency, Fisheri
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