Synergy between nanozymes and natural enzymes on the hybrid MoS 2 nanosheets/graphite microfiber for enhanced voltammetr
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ORIGINAL PAPER
Synergy between nanozymes and natural enzymes on the hybrid MoS2 nanosheets/graphite microfiber for enhanced voltammetric determination of hydrogen peroxide Jian Zhang 1,2 & Dong Han 3 & Yanhui Wang 2 & Longwei Wang 1 & Xuanyu Chen 1 & Xiran Qiao 4 & Xin Yu 1 Received: 10 January 2020 / Accepted: 23 April 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A biosensor for hydrogen peroxide (H2O2) has been developed based on the use of MoS2 nanosheets and graphite that are assembled to form a microfiber hybrid structure. The MoS2 nanosheets are synthesized in situ on a graphite microfiber. The chemical composition and surface morphology of the microfiber hybrid structure has been characterized. The microfiber is shown to display peroxidase-mimicking activity. In the next step, horseradish peroxidase, methylene blue, and chitosan are co-immobilized on the microfiber electrode. The use of MoS2 nanosheets warrants high electrochemical activity of immobilized enzyme on the electrode surface. The modified microfiber electrode, best operated at a voltage of − 0.3 V (vs. Ag/AgCl), can be used to sense H2O2 with a linear response in the 0.1 to 90 μM concentration range and with a determination limit of 30 nM (at S/N = 3). The good response is attributed to the synergistic enhancement of the synthetic nanozymes (few-layered MoS2 nanosheets) and immobilized natural horseradish peroxidase (HRP). Keywords MoS2 nanosheets/graphite microfiber . H2O2 determination . Electrochemical sensor . Peroxidase-like activity
Introduction The accurate determination of hydrogen peroxide (H2O2) plays an important role in many areas including industrial production, clinical control, and environmental conservation [1]. According to the US Food and Drug Administration, the residual amount of H2O2 in food should not exceed 0.5 ppm [2]. In living organisms, H2O2 also plays an important role as a signaling Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04299-3) contains supplementary material, which is available to authorized users. * Xin Yu [email protected] 1
Institute for Advanced Interdisciplinary Research (iAIR), University of Jinan, Jinan 250022, People’s Republic of China
2
Institut Charles Gerhardt de Montpellier, UMR 5253, Université de Montpellier, CNRS, ENSCM, 34095 Montpellier Cedex 5, France
3
Université de Lyon, Ecole Centrale de Lyon, UMR CNRS 5270, Institut des Nanotechnologies de Lyon, UMR CNRS 5270, 69130 Ecully, France
4
School of Textile Science and Engineering, Tiangong University, Tianjin 300387, People’s Republic of China
molecule in a variety of biological regulation processes, such as immune cell activation, vascular remodeling, apoptosis, and stomatal closure [3]. It is necessary to determine H2O2, as low as possible. Electrochemistry is considered as a convenient method for H2O2 determination, because it has good sensitivity, time efficiency, and low cost [4, 5]. Horseradish peroxidase (HRP) is the most common enzyme
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