Systemic treatment with alpha-tocopherol and/or sodium selenite decreases the progression of experimental periodontitis
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ORIGINAL ARTICLE
Systemic treatment with alpha-tocopherol and/or sodium selenite decreases the progression of experimental periodontitis Nurgül Bas 1 & Nezahat Arzu Kayar 2 Nilgün Özlem Alptekin 6
&
Z. Füsun Baba 3 & Mustafa Cihat Avunduk 4 & Seyfullah Haliloğlu 5 &
Received: 10 May 2020 / Accepted: 10 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Objective To investigate the effects of sodium selenite (Se) and/or α-tocopherol (αT) applications on the alveolar bone loss (ABL), the number of gingival collagen fibers, inducible nitric oxide synthase (iNOS)+ and CD95+ cell numbers, and serum cytokine concentrations in experimental periodontitis in rats. Materials and methods Forty Sprague Dawley rats were divided into four groups of ten as follows: group A: Se group, group B: αT group, group C: Se and αT combined group, and group D: control group (intraperitoneal (IP) saline injection applied). Using the image analysis method in the connective tissue under the connective epithelium, the numbers of iNOS, CD95 positive cells, and collagen fibers were counted. ELISA kits were used to test the concentrations of serum interleukin (IL)-1β, IL-6, and IL-4. Results The combination of Se and αT (group C) suppressed ABL compared with the control group (group D) (P < 0.05). In group A (Se), the number of iNOS+ cells was smaller than in group D (P < 0.05). Conclusion Se has been concluded to inhibit inflammation of the gum due to iNOS. Se and αT can have a remarkable important role in preventing alveolar bone loss, and particularly in combination. Clinical relevance Se and/or αT application may be useful in preventing the destruction of periodontal tissue and treatment of periodontal disease. Keywords Animal model . Experimental periodontitis . α-Tocopherol . Sodium selenite . CD95 . iNOS . IL-1β . IL-6 . IL-4
Introduction Periodontal disease is an inflammatory disorder that damages tissue through the complex interactions between periodontopathic bacteria and host defense systems. * Nezahat Arzu Kayar [email protected]
Neutrophils are the first leukocytes to migrate to the inflammatory site and play an important role in the management of periodontal disease. Monocytes and polymorphonuclear leucocytes’ antimicrobial activities are characterized as either oxygen-dependent or oxygen-independent systems [1]. 1
Kayseri Nimet Bayraktar Oral and Dental Health Center, Republic of Turkey Ministry of Health, Kayseri, Turkey
2
Department of Periodontology, Faculty of Dentistry, Akdeniz University, 07058 Antalya, Turkey
3
Patology Laboratory, Acıbadem International Hospital, İstanbul, Turkey
4
Department of Pathology, Meram Faculty of Medicine, Necmettin Erbakan University, Konya, Turkey
5
Department of Biochemistry, Faculty of Veterinary, Selcuk University, Konya, Turkey
6
Department of Periodontology, Faculty of Dentistry, Başkent University, Ankara, Turkey
Nurgül Bas [email protected] Z. Füsun Baba [email protected] Mustafa Cihat Avunduk [email protected] Seyfullah Ha
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