The Barcoding Table of Animal Species (BaTAnS): a new tool to select appropriate methods for animal species identificati

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The Barcoding Table of Animal Species (BaTAnS): a new tool to select appropriate methods for animal species identification using DNA barcoding Nele Matthes1   · Klaus Pietsch2 · Anke Rullmann3 · Gabriele Näumann4 · Bert Pöpping5 · Kathrin Szabo6 Received: 31 January 2020 / Accepted: 19 July 2020 © Springer Nature B.V. 2020

Abstract Food and feed products derived from animal materials have a long history of being adulterated. Methods for the identification of animal samples based on DNA barcoding are very potent tools to reveal species substitution. Since numerous DNA barcoding methods are available for different taxa, it is challenging to choose an appropriate and verified method for each sample in question. To overcome this obstacle the working group “Molecular biological methods for plant and animal species differentiation” developed the “Barcoding Table of Animal Species”. This working group is established through the German food and feed law and is mandated to validate standard methods, especially for the official food and feed control laboratories in Germany. In this paper, a collection of currently available and verified DNA barcoding methods for the identification of animal species is presented as a Microsoft E ­ xcel® file—“The Barcoding Table of Animal Species (BaTAnS)”. It consists of several components: The method collection, the results collection and a section for reporting new entries and problems. It is focusing on the validity and applicability of DNA barcoding methods to test food and feed products for correct species declaration. Each method is listed with its reference and is verified by at least two laboratories for their applicability. Since additional information will be available in future, this table will be updated regularly. The BaTAnS is an easy tool that helps to choose the right verified method to identify a certain specimen to taxon, genus or species level in food samples. Keywords  Food fraud/authenticity · DNA barcoding · Environment protection/CITES · Species identification

Introduction Electronic supplementary material  The online version of this article (https​://doi.org/10.1007/s1103​3-020-05675​-1) contains supplementary material, which is available to authorized users. * Nele Matthes [email protected] 1



State Office for Agriculture, Food Safety and Fishery Mecklenburg-Vorpommern, Thierfelderstraße 18, 18059 Rostock, Germany

2



State Institute for Chemical and Veterinary Analysis Freiburg, Bissierstr. 5, 79114 Freiburg, Germany

3

State Institute for Chemical and Veterinary Analysis Karlsruhe, Weissenburgerstr. 3, 76187 Karlsruhe, Germany

4

Institute for Hygiene and Environment Hamburg, Markmannstr. 129b, 20539 Hamburg, Germany

5

FOCOS – Food Consulting Strategically, Zum Kälterhaus 6b, 63755 Alzenau, Germany

6

Federal Office of Consumer Protection and Food Safety, Mauerstr. 39‑42, 10117 Berlin, Germany



Animal products have a long and global history of being adulterated. There are several types of adulterations, which include t