The Biomedical Use of Rescaling Procedures in Optical Biopsy and Optical Molecular Imaging

Using fluorescence imaging in biomedicine, the analysis of laser-induced autofluorescences shows comparable strong intensity distortion for endogenous NADH in the UV and synthesized markers in the NIR range due to tissue optics. Rescaling, taking into acc

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O. Wolfbeis Editor-in-Chief

Springer-Verlag Berlin Heidelberg GmbH

Ruud Kraayenhof • Antonie J. W. G. Visser • Hans C. Gerritsen (Eds.)

Fluorescence Spectroscopy, Imaging and Probes New Tools in Chemical, Physical and Life Sciences

With 175 Figures

,

Springer

Fluorescence spectroscopy, fluorescence imaging and fluorescent probes are indispensible tools in numerous fields of modern medicine and science, including molecular biology, biophysics, biochemistry, clinical diagnosis and analytical and environmental chemistryo Applications stretch from spectroscopy and sensor technology to microscopy and imaging, to single molecule detection, to the development of novel fluorescent probes, and to proteomics and genomicso The Springer Series on Fluorescence aims at publishing state-of-the-art artic1es that can serve as invaluable tools for both practitioners and researchers being active in this highly interdisciplinary fieldo The carefully edited collection of papers in each volume will give continuous inspiration for new research and will point to exciting new trendso

- Springer WWW home page: http://www.springer.de

ISSN 1617-1306 ISBN 978-3-642-62732-3 Library ofCongress Cataloging-in-Publication Data applied for Die Deutsche Bibliothek - Cip-Einheitsaufnahme Fluorescence spectroscopy, imaging and probes : new tools in chemical, physical and life sciences I Ruud Kraayenhof o.. (ed.). - Berlin; Heidelberg; New York; Barcelona ; Hong Kong ; London ; Milan ; Paris; Tokyo: Springer, 2002 (Springer series on fluorescence ; VoI. 2) ISBN 978-3-642-62732-3 ISBN 978-3-642-56067-5 (eBook) DOI 10.1007/978-3-642-56067-5

This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilm or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the provisions of the German Copyright Law of September 9, 1965, in its current version, and permission for use must always be obtained from Springer-Verlago Violations are liable for prosecution under the German Copyright Law. http://www.springer.de © Springer-Verlag Berlin Heidelberg 2002 Originally published by Springer-Verlag Berlin Heidelberg New York in 2002 Softcover reprint of the hardcover 1st edition The use of registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Typesetting: Data delivered by editors Cover: design & production, Heidelberg Printed on acid-free paper SPIN: 10993422

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Editor-in-Chief Professor Dr. Otto Wolfbeis University of Regensburg Institute of Analytical Chemistry, Chemo- and Biosensors 93040 Regensburg Germany

Volume Editor Professor Ruud Kraayenhof Institute of Molecular Biological Sciences, Amsterdam Vrije Universiteit De