The capping pheromones and putative biosynthetic pathways in worker and drone larvae of honey bees Apis mellifera
- PDF / 723,381 Bytes
- 11 Pages / 467.716 x 680.315 pts Page_size
- 0 Downloads / 187 Views
Original article
The capping pheromones and putative biosynthetic pathways in worker and drone larvae of honey bees Apis mellifera Qiu-Hong QIN1,2 , Xu-Jiang HE1 , Andrew B. BARRON3 , Lei GUO4 , Wu-Jun JIANG1 , Zhi-Jiang ZENG1 1
Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045 Jiangxi, People’s Republic of China Guangxi Liuzhou Animal Husbandry and Veterinary School, Liuzhou 545003 Guangxi, People’s Republic of China 3 Department of Biological Sciences, Macquarie University, North Ryde, NSW 2109, Australia 4 Research and Development Centre, China Tobacco Jiangxi Industrial Co., LTD., Nanchang 330096 Jiangxi, People’s Republic of China 2
Received 24 January 2019 – Revised 28 June 2019 – Accepted 1 September 2019
Abstract – In honey bees (Apis mellifera ), methyl palmitate (MP), methyl oleate (MO), methyl linoleate (ML), and methyl linolenate (MLN) are important pheromone components of the capping pheromones triggering the capping behavior of worker bees. In this study, we compared the amounts of these four pheromone components in the larvae of workers and drones, prior to be capped, in the process of being capped and had been capped. The amounts of MP, MO, and MLN peaked at the capping larval stage, and ML was highest at capped larvae in worker larvae, whereas in drone larvae, the amounts of the four pheromone components were higher overall and increased with aging. Furthermore, we proposed de novo biosynthetic pathways for MP, MO, ML, and ML, from acetyl-CoA. Besides, stable isotope tracer 13C and deuterium were used to confirm that these capping pheromone components were de novo synthesized by larvae themselves rather than from their diets. honey bee / larvae / capping / pheromones / pathways
1. INTRODUCTION Honey bees have been instrumental in revealing both the significance and the complexity of pheromonal communication systems. PheroElectronic supplementary material The online version of this article (https://doi.org/10.1007/s13592-019-00686-9) contains supplementary material, which is available to authorized users.
Corresponding author: Z. Zeng, [email protected] Qiu-Hong Qin and Xu-Jiang He contributed equally to this work. All sequencing data has been submitted to GenBank under accession numbers SRR4051855, SRR4051820, bioproject PRJNA339509 Handling editor: Klaus Hartfelder
mones help to coordinate a colony’s collective behaviour, such as foraging, defensive behaviour, and brood-rearing activity (Free and Winder 1983; Free 1987; Vallet et al. 1991; Breed et al. 2004; Hunt 2007; Stout and Goulson 2001; Slessor et al. 2005; Maisonnasse et al. 2010). The subtleties and complexities of bee pheromonal communication are perhaps best illustrated by the pheromonal communication between workers and developing larvae. Honey bee larvae are entirely dependent on their attendant adult workers. Larvae release capping pheromone components, form their salivary glands containing ten methyl or ethyl fatty acid esters (Le Conte et al. 1989, 1990, 2006; Trouiller et al. 1991). The compositio
Data Loading...
